Band 3/AE 1 Monoklonaler Antikörper

Band 3/AE 1 Monoklonal Antikörper für IHC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human und mehr (1)

Anwendung

IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

1E5C1

Kat-Nr. : 66984-1-Ig

Synonyme

band 3, band 3/ AE1, SLC4A1, 1E5C1, AE 1

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  IHC staining of human placenta using 66984-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66984-1-Ig (band 3/ AE1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 66984-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66984-1-Ig (band 3/ AE1 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human placenta using 66984-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Band 3/AE 1 antibody (66984-1-Ig, Clone: 1E5C1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human placenta using 66984-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Band 3/AE 1 antibody (66984-1-Ig, Clone: 1E5C1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Plazenta-Gewebe

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:5000-1:20000
• Immunfluoreszenz (IF)-P: IF-P : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below

Produktinformation

66984-1-Ig bindet in IHC, IF-P, ELISA Band 3/AE 1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Band 3/AE 1 fusion protein Ag28058
• Vollständiger Name: solute carrier family 4, anion exchanger, member 1 (erythrocyte membrane protein band 3, Diego blood group)
• Berechnetes Molekulargewicht: 911 aa, 102 kDa
• GenBank-Zugangsnummer: BC101570
• Gene symbol: band 3
• Gene ID (NCBI): 6521
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Anion exchanger 1 (AE1), also known as band 3, is a 95-100 kDa transmembrane glycoprotein which is abundantly expressed in erythrocyte plasma membrane and mediates the electroneutral exchange of Cl- and HCO3-. It contains a 52-55 kDa C-terminal membrane crossing domain and a 43 kDa N-terminal cytoplasmic domain. Degradation of band 3 protein occurred during erythrocyte aging. The oxidative stress can also activate the proteolysis of band 3 through caspases. This antibody is expected to recognize the intact band 3 (95-100 kDa) and various degradation products (38-60 kDa). (PMID: 22890269, 29240292, 28130418)

Publikationen

Species	Application	Title
mouse	IHC	Neuron; Single-cell dissection of the human blood-brain barrier and glioma blood-tumor barrier; Authors - Yuan Xie