acetylated Tubulin(Lys40) Monoklonaler Antikörper

acetylated Tubulin(Lys40) Monoklonal Antikörper für IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Hund, Maus, Ratte und mehr (1)

Anwendung

IF

Konjugation

CoraLite®594 Fluorescent Dye

Publikationen

3

CloneNo.

7E5H8

Kat-Nr. : CL594-66200

Synonyme

acetylated alpha tubulin, Acetylated tubulin, acetylated Tubulin (Lys40), acetyl-a-tubulin, alpha tubulin, Alpha tubulin 3, B ALPHA 1, LIS3, TUBA1A, TUBA3, Tubulin alpha 1A chain, Tubulin alpha 3 chain, Tubulin B alpha 1, tubulin, alpha 1a

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Galerie der Validierungsdaten

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  IF Staining of MDCK using CL594-66200

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using CL594-66200 (acetylated Tubulin(Lys40) antibody) at dilution of 1:100.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: MDCK-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 3 publications below

Produktinformation

CL594-66200 bindet in IF acetylated Tubulin(Lys40) und zeigt Reaktivität mit Hausschwein, human, Hund, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Hund, Maus, Ratte
• In Publikationen genannte Reaktivität: Zebrafisch
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: tubulin, alpha 1a
• Berechnetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: NM_006009
• Gene symbol: TUBA1A
• Gene ID (NCBI): 7846
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tubulin, composed of heterodimers of alpha and beta tubulin, is the mainly component of microtubules which play important roles in cell motility, mitosis, and intracellular vesicle transport. Both alpha and beta tubulin undergo several posttranslational modifications such as polyglutamylation and acetylation/deacetylation. Tubulin acetylation occurs on lysine-40 at the N-terminal of alpha tubulin and is conserved across species. The histone deacetylase HDAC6 and SIRT2 has been identified as tubulin deacetylases. Reversible acetylation of alpha tubulin may be implicated in regulating microtubule stability, cell motility, and axon regeneration. The level of acetylated tubulin has been linked to epithelial malignancies and sensitivity to chemotherapy. In addition, acetylated tubulin has been widely used as a marker for primary cilia. This antibody is specific to the acetylated tubulin; it does not recognize non-acetylated tubulin. (24268707, 23881549) And this anitbody is CL594(Ex/Em 593 nm/614 nm) conjugated.

Publikationen

Species	Application	Title
	IF	J Neurosci; Comparison of ciliary targeting of two rhodopsin-like GPCRs: role of C-terminal localization sequences in relation to cilium type.; Authors - Abhishek Chadha
	IF	Curr Biol; Cones and cone pathways remain functional in advanced retinal degeneration; Authors - Erika M Ellis
zebrafish	IF	Cell Biosci; IK is essentially involved in ciliogenesis as an upstream regulator of oral-facial-digital syndrome ciliopathy gene, ofd1; Authors - Hye In Ka