ZNF746 Monoklonaler Antikörper

ZNF746 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

3A12C5

Kat-Nr. : 67463-1-Ig

Synonyme

FLJ31413, PARIS, zinc finger protein 746, ZNF746

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 67463-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67463-1-Ig (ZNF746 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of LNCaP using 67463-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67463-1-Ig (ZNF746 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, LNCaP-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67463-1-Ig bindet in WB, ELISA ZNF746 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ZNF746 fusion protein Ag21582
• Vollständiger Name: zinc finger protein 746
• Berechnetes Molekulargewicht: 644 aa, 69 kDa
• Beobachtetes Molekulargewicht: 85-90 kDa
• GenBank-Zugangsnummer: BC068505
• Gene symbol: ZNF746
• Gene ID (NCBI): 155061
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ZNF746, also named as PARIS, which belongs to the krueppel C2H2-type zinc-finger protein family. ZNF746 mainly localizes to the cytoplasm and probably translocates to the nucleus to repress selected genes. ZNF746 as a transcription repressor that specifically binds to the 5'-TATTTT[T/G]-3' consensus sequence on promoters and repress transcription of PGC-1-alpha (PPARGC1A), thereby playing a role in regulation of neuron death. ZNF746 may act as a downstream effector of PRKN and contribute to neurodegeneration in Parkinson disease cases caused by defects in PRKN: its accumulation due to the absence of PRKN, followed by up-regulation of PPARGC1A, could lead to the selective loss of dopamine neurons in the substantia nigra(PMID: 28122242). The calculated molecular weight of ZNF746 is 69 kDa, but Phosphorylation of ZNF746 is about 80-85 kDa (PMID: 28122242).