ZNF395 Polyklonaler Antikörper

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• KD/KO Validated

ZNF395 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 11759-1-AP

Synonyme

HD-regulating factor 2, HDBP-2, HDBP2, HDBP 2, HD regulating factor 2

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Galerie der Validierungsdaten

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  WB analysis of human brain using 11759-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11759-1-AP (ZNF395 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 11759-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11759-1-AP (ZNF395 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11759-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11759-1-AP (ZNF395 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 11759-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11759-1-AP (ZNF395 antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of HepG2 using 11759-1-AP

  Immunofluorescent analysis of HepG2 cells using 11759-1-AP (ZNF395 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Produktinformation

11759-1-AP bindet in WB, IHC, IF/ICC, ELISA ZNF395 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ZNF395 fusion protein Ag2355
• Vollständiger Name: zinc finger protein 395
• Berechnetes Molekulargewicht: 513 aa, 55 kDa
• Beobachtetes Molekulargewicht: 62 kDa, 72 kDa
• GenBank-Zugangsnummer: BC001237
• Gene symbol: ZNF395
• Gene ID (NCBI): 55893
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ZNF395 is a transcription factors binding to a 7bp GC rich sequence which resides in triplicate at intervals of 13bp within and proximal to the -20bp direct repeat sequences of the Htt promoter. It can bind to regulatory regions in papillomaviruses (PV) and subsequently called the protein papillomavirus binding factor (PBF) [PMID: 11853404]. It contains conserved regions CR1, CR2 and CR3, a domain rich in serines and prolines and have the potential to form a zinc-finger structure, and the C-terminal CR3 is responsible for DNA-binding [PMID: 17897615].

Publikationen

Species	Application	Title
human	WB	Onco Targets Ther; Dysregulation of Zinc Finger Protein 395 Contributes to the Pathogenesis of Chondrosarcoma.; Authors - Changbao Chen
human	WB,IHC	Pathobiology; Downregulation of ZNF395 Drives Progression of Pancreatic Ductal Adenocarcinoma through Enhancement of Growth Potential.; Authors - Shusaku Kurogi
human	WB	J Exp Clin Cancer Res; HnRNP A1 - mediated alternative splicing of CCDC50 contributes to cancer progression of clear cell renal cell carcinoma via ZNF395.; Authors - Guoliang Sun ; KD Validated