ZNF238/ZBTB18 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ZNF238/ZBTB18 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, RIP, IHC, CoIP, chIP, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Kat-Nr. : 12714-1-AP

Synonyme

58 kDa repressor protein, C2H2 171, RP58, TAZ 1, TAZ1, Transcriptional repressor RP58, ZBTB18, ZFP238, zinc finger protein 238, Zinc finger protein C2H2 171, ZNF238

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Galerie der Validierungsdaten

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  WB analysis using 12714-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12714-1-AP (ZNF238 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 12714-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12714-1-AP (ZNF238 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 12714-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12714-1-AP (ZNF238 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 12714-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12714-1-AP (ZNF238 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 12714-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12714-1-AP (ZNF238 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12714-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12714-1-AP (ZNF238 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12714-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12714-1-AP (ZNF238 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12714-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12714-1-AP (ZNF238 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 12714-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12714-1-AP (ZNF238 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 12714-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12714-1-AP (ZNF238 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 12714-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12714-1-AP (ZNF238 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 12714-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12714-1-AP (ZNF238 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 12714-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12714-1-AP (ZNF238 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 12714-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12714-1-AP (ZNF238 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 12714-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12714-1-AP (ZNF238 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of rat brain using 12714-1-AP

  IP result of anti-ZNF238/ZBTB18 (IP:12714-1-AP, 4ug; Detection:12714-1-AP 1:3000) with rat brain tissue lysate 1880 ug.

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  IHC staining of mouse brain using 12714-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12714-1-AP (ZNF238 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse brain using 12714-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12714-1-AP (ZNF238 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse skin using 12714-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 12714-1-AP (ZNF238 antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 12714-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 12714-1-AP (ZNF238 antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Cerebellum-Gewebe, Mausherzgewebe, Maushirngewebe, Mausnierengewebe, Rattenherzgewebe
• Erfolgreiche IP: Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, Maushautgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 7 publications below
• CoIP: See 2 publications below
• ChIP: See 3 publications below
• RIP: See 1 publications below

Produktinformation

12714-1-AP bindet in WB, IP, IF, RIP, IHC, CoIP, chIP, ELISA ZNF238/ZBTB18 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ZNF238/ZBTB18 fusion protein Ag3406
• Vollständiger Name: zinc finger protein 238
• Berechnetes Molekulargewicht: 531 aa, 59 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC036677
• Gene symbol: ZNF238/ZBTB18
• Gene ID (NCBI): 10472
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ZNF238 is a member of the BTB/POZ-ZF protein family, which involve in development and cancer formation, for example BCL-6, PLZF, and HIC-1. It's a transcriptional repressor involve in myogenesis and brain development. By directly repressing the expression of two skeletal myogenesis inhibitors, ID2 and ID3, ZNF238 plays a key role in myogenesis. It can control cell division of progenitor cells and regulating the survival of postmitotic cortical neurons. Besides, ZNF238 involves in the organization of nuclear chromosomes, for its specific binding to the consensus DNA sequence that contains the E box core, and recruiting chromatin remodeling multi-protein complex. ZNF238 proteins has apparent molecular masses of 60 and 48 kD. Specific binding is found for a 60-kDa band which corresponds to the full length of RP58 protein. In addition, a 48-kDa band, thought to be the truncated form 2 is detected (PMID: 9756912).

Publikationen

Species	Application	Title
mouse	chIP	Neuron; FOXG1 Orchestrates Neocortical Organization and Cortico-Cortical Connections.; Authors - Francesca Cargnin
mouse	IF	PLoS Biol; Olig2 and Hes regulatory dynamics during motor neuron differentiation revealed by single cell transcriptomics.; Authors - Andreas Sagner
mouse	IHC	Cereb Cortex; The Zinc Finger Transcription Factor RP58 Negatively Regulates Rnd2 for the Control of Neuronal Migration During Cerebral Cortical Development.; Authors - Heng Julian Ik-Tsen JI
human	WB,CoIP	iScience; Calpain-mediated cleavage generates a ZBTB18 N-terminal product that regulates HIF1A signaling and glioblastoma metabolism.; Authors - Anie P Masilamani
MOUSE	IF	Neural Dev; Rp58 and p27(kip1) coordinate cell cycle exit and neuronal migration within the embryonic mouse cerebral cortex.; Authors - Olivier Clément
human	WB,RIP	MedComm (2020); LINC02774 inhibits glycolysis in glioma to destabilize HIF-1α dependent on transcription factor RP58; Authors - Yuanbing Chen