ZMYND8 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ZMYND8 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, IP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 11633-1-AP

Synonyme

CTCL associated antigen se14 3, KIAA1125, PRKCBP1, PRO2893, RACK7, ZMYND8

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 11633-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 11633-1-AP (ZMYND8 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  WB analysis of HeLa using 11633-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11633-1-AP (ZMYND8 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 11633-1-AP

  IP result of anti-ZMYND8 (IP:11633-1-AP, 3ug; Detection:11633-1-AP 1:300) with HeLa cells lysate 920ug.

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  IHC staining of human lung cancer using 11633-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11633-1-AP (ZMYND8 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 11633-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11633-1-AP (ZMYND8 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 11633-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11633-1-AP (ZMYND8 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11633-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11633-1-AP (ZMYND8 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, HeLa-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• ChIP: See 1 publications below

Produktinformation

11633-1-AP bindet in WB, IHC, IF, IP, ChIP, ELISA ZMYND8 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ZMYND8 fusion protein Ag2207
• Vollständiger Name: zinc finger, MYND-type containing 8
• Berechnetes Molekulargewicht: 1186 aa, 132 kDa
• Beobachtetes Molekulargewicht: 150 kDa
• GenBank-Zugangsnummer: BC030721
• Gene symbol: ZMYND8
• Gene ID (NCBI): 23613
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Regulation of neuronal gene expression is essential to nervous system development. REST (RE1-silencing transcription factor) regulates neuronal gene expression by interacting with a group of corepressor proteins including REST corepressors (RCOR). ZMYND8 bind to ROCR2 and can function as transcriptional repressors. It is co-expressed with RCOR2 in the nervous system and overexpression of ZMYND8 inhibits neural differentiation. [PMID:20331974]

Publikationen

Species	Application	Title
mouse,human		Mol Cell; The ZMYND8-regulated mevalonate pathway endows YAP-high intestinal cancer with metabolic vulnerability.; Authors - Qiang Pan ; KO Validated
human	WB	Mol Cancer; LncRNA TROJAN promotes proliferation and resistance to CDK4/6 inhibitor via CDK2 transcriptional activation in ER+ breast cancer.; Authors - Xi Jin ; KD Validated
human	WB	Cancer Res; KDM5C-mediated recruitment of BRD4 to chromatin regulates enhancer activation and BET inhibitor sensitivity; Authors - Yulong Qiang
human	WB	Mol Cell; Basis of gene-specific transcription regulation by the Integrator complex; Authors - Kevin Sabath
human	WB,IHC,IF	Sci Adv; The endogenous retrovirus-derived long noncoding RNA TROJAN promotes triple-negative breast cancer progression via ZMYND8 degradation.; Authors - Xi Jin
mouse	ChIP	J Tissue Eng; Mesenchymal stem cells overexpressing XIST induce macrophage M2 polarization and improve neural stem cell homeostatic microenvironment, alleviating spinal cord injury; Authors - Dan Zhu