ZFYVE16 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ZFYVE16 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 13118-2-AP

Synonyme

Zinc finger FYVE domain-containing protein 16, KIAA0305, Endosome-associated FYVE domain protein, Endofin

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Galerie der Validierungsdaten

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  WB analysis using 13118-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13118-2-AP (ZFYVE16 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 13118-2-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13118-2-AP (ZFYVE16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 13118-2-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13118-2-AP (ZFYVE16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 13118-2-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13118-2-AP (ZFYVE16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13118-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13118-2-AP (ZFYVE16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 13118-2-AP

  IP result of anti-ZFYVE16 (IP:13118-2-AP, 5ug; Detection:13118-2-AP 1:500) with HeLa cells lysate 2000ug.

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  IHC staining of human kidney using 13118-2-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 13118-2-AP (ZFYVE16 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human kidney using 13118-2-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 13118-2-AP (ZFYVE16 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 13118-2-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13118-2-AP (ZFYVE16 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 13118-2-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13118-2-AP (ZFYVE16 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 13118-2-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 13118-2-AP (ZFYVE16 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 13118-2-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 13118-2-AP (ZFYVE16 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 13118-2-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 13118-2-AP (ZFYVE16 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 13118-2-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 13118-2-AP (ZFYVE16 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 13118-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ZFYVE16 antibody (13118-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). CL594-Phalloidin (red). DAPI (blue)

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, humanes Plazenta-Gewebe, Mausnierengewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Herzgewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Produktinformation

13118-2-AP bindet in WB, IHC, IF/ICC, IP, ELISA ZFYVE16 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ZFYVE16 fusion protein Ag3811
• Vollständiger Name: zinc finger, FYVE domain containing 16
• Berechnetes Molekulargewicht: 1539 aa, 169 kDa
• Beobachtetes Molekulargewicht: 230-250 kDa
• GenBank-Zugangsnummer: BC032227
• Gene symbol: ZFYVE16
• Gene ID (NCBI): 9765
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Endofin (also known as ZFYVE16) is an endosomal FYVE domain protein implicated in regulating membrane trafficking. Endofin has been shown to localize to early endosomes. It co-localizes with SARA but does not associate with it or Smad2 and does not behave like SARA in affecting TGF-signaling. Northern blot analysis showed ZFYVE16 was widely expressed with high levels in kidney, placenta and lung. Overexpression of Endofin causes endosome aggregation. The gene of ZFYVE16 maps to chromosome 5q14, and encodes a 1539-amino acid protein with a molecular mass of 169 kDa. In addition, endogenous Endofin can also be detected as a band of 230-250 kDa. (PMID: 11546807)

Publikationen

Species	Application	Title
zebrafish	IF	PLoS Genet; The Lowe Syndrome Protein OCRL1 Is Required for Endocytosis in the Zebrafish Pronephric Tubule.; Authors - Francesca Oltrabella
human	WB	Structure; Structural Basis for Specific Interaction of TGFβ Signaling Regulators SARA/Endofin with HD-PTP.; Authors - Deepankar Gahloth ; KD Validated
human	WB	iScience; Endofin is required for HD-PTP and ESCRT-0 interdependent endosomal sorting of ubiquitinated transmembrane cargoes.; Authors - Jalal M Kazan ; KD Validated
human	WB	FEBS Lett; SARA is dispensable for functional TGF-β signaling.; Authors - Bakkeb? Maren M ; KD Validated