ZEB2 Polyklonaler Antikörper

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• KD/KO Validated

ZEB2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (4)

Anwendung

WB, IF, IHC, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

64

Kat-Nr. : 14026-1-AP

Synonyme

KIAA0569, SIP 1, SIP1, Smad interacting protein 1, SMADIP1, ZEB2, ZFHX1B, ZFX1B

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Galerie der Validierungsdaten

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  WB analysis using 14026-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14026-1-AP (ZEB2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14026-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14026-1-AP (ZEB2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14026-1-AP

  WB result of ZEB2 antibody (14026-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZEB2 transfected HepG2 cells.

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  IHC staining of human lung cancer using 14026-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14026-1-AP (ZEB2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 14026-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 14026-1-AP (ZEB2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 14026-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14026-1-AP (ZEB2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe, humanes Lungenkarzinomgewebe, humanes Nierenzellkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 58 publications below
• IHC: See 5 publications below
• IF: See 4 publications below
• ChIP: See 1 publications below

Produktinformation

14026-1-AP bindet in WB, IF, IHC, ChIP, ELISA ZEB2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Huhn, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ZEB2 fusion protein Ag5171
• Vollständiger Name: zinc finger E-box binding homeobox 2
• Berechnetes Molekulargewicht: 136 kDa
• Beobachtetes Molekulargewicht: 150-170 kDa
• GenBank-Zugangsnummer: BC060819
• Gene symbol: ZEB2
• Gene ID (NCBI): 9839
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Zinc finger E-box-binding homeobox (ZEB2) is a key transcription factor that acts as a multifunctional regulator during nervous system development. ZEB2 contains two zinc finger domains and a homeodomain-like sequence and interacts with the TGF-ß superfamily signaling regulators, Smads, to regulate the expression of their downstream genes. ZEB2 is expressed in the developing neural tube, as well as in neural crest cells, the hippocampus and the cerebral cortex. ZEB2 has previously been implicated in EMT, cell-cycle progression, apoptosis and senescence. ZEB2 was overexpressed in bladder, ovarian, stomach, pancreatic and squamous cell carcinoma, in the intestinal subtype of stomach cancers, and at the invasive front of CRC where EMT is most prominen. ZEB2 also mediates cell-fate decision in neuronal, T cells and hematopoietic stem cells. The calculated molecular weight of ZEB2 is 136 kDa, but we find the 95 kDa band in some publication(PMID: 27659015 )

Publikationen

Species	Application	Title
mouse	IF	Nat Commun; Symmetry breaking of tissue mechanics in wound induced hair follicle regeneration of laboratory and spiny mice.; Authors - Hans I-Chen Harn
human	WB,IF	Cell Death Differ; Regulation of Semaphorin3A in the process of cutaneous wound healing.; Authors - Yang Zheng
human	WB	Cancer Lett; A novel antagonist of the CCL5/CCR5 axis suppresses the tumor growth and metastasis of triple-negative breast cancer by CCR5-YAP1 regulation; Authors - Ling Chen
human	WB	Oncotarget; Pygopus-2 promotes invasion and metastasis of hepatic carcinoma cell by decreasing E-cadherin expression.; Authors - Sheng Zhang
human	WB	Cell Death Dis; PIM1 mediates epithelial-mesenchymal transition by targeting Smads and c-Myc in the nucleus and potentiates clear-cell renal-cell carcinoma oncogenesis.; Authors - Bin Zhao
human	WB	J Gastroenterol; LncRNA n339260 functions in hepatocellular carcinoma progression via regulation of miRNA30e-5p/TP53INP1 expression.; Authors - Tieju Liu