ZBTB20 Polyklonaler Antikörper

ZBTB20 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 23987-1-AP

Synonyme

DPZF, HOF, ODA 8S, ZBTB20, Zinc finger protein 288, ZNF288

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Galerie der Validierungsdaten

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  WB analysis using 23987-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23987-1-AP (ZBTB20 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 23987-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 23987-1-AP (ZBTB20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 23987-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 23987-1-AP (ZBTB20 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 23987-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23987-1-AP (ZBTB20 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 23987-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 23987-1-AP (ZBTB20 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 23987-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 23987-1-AP (ZBTB20 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 23987-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23987-1-AP (ZBTB20 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 23987-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 23987-1-AP (ZBTB20 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 23987-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23987-1-AP (ZBTB20 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 23987-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23987-1-AP (ZBTB20 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of mouse brain using 23987-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using ZBTB20 antibody (23987-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HEK-293-Zellen, Jurkat-Zellen, L02-Zellen, Maushirngewebe, Mauslebergewebe, Rattenlebergewebe, RAW 264.7-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

23987-1-AP bindet in WB, IHC, IF, ELISA ZBTB20 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ZBTB20 fusion protein Ag21140
• Vollständiger Name: zinc finger and BTB domain containing 20
• Berechnetes Molekulargewicht: 668 aa, 73 kDa
• Beobachtetes Molekulargewicht: 73 kDa
• GenBank-Zugangsnummer: BC029041
• Gene symbol: ZBTB20
• Gene ID (NCBI): 26137
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. ZBTB20 (zinc finger and BTB domain containing 20), also known as HOF, DPZF, ODA-8S or ZNF288, is a 741 amino acid protein that localizes to the nucleus and contains one BTB (POZ) domain and five C2H2-type zinc fingers. Expressed in thymus, spleen, lymph node and fetal liver, ZBTB20 exists as either a monomer or a homodimer that is thought to function as a transcription factor, playing a role in hematopoiesis, oncogenesis and immune responses. Multiple isoforms of ZBTB20 exist due to alternative splicing events.

Publikationen

Species	Application	Title
	IF	Cell Prolif; In vivo development and single-cell transcriptome profiling of human brain organoids.; Authors - Shichao Huang
mouse	WB,IHC	J Inflamm Res; Aberrant Fluid Shear Stress Contributes to Articular Cartilage Pathogenesis via Epigenetic Regulation of ZBTB20 by H3K4me3.; Authors - Yu Jin
mouse	WB	Wellcome Open Res; The integration site of the APP transgene in the J20 mouse model of Alzheimer's disease.; Authors - Justin L Tosh
rat	WB	Cell Rep; Identification of dynamic RNA-binding proteins uncovers a Cpeb4-controlled regulatory cascade during pathological cell growth of cardiomyocytes.; Authors - Eva Riechert
mouse	WB	Transpl Immunol; ZBTB20 regulates cardiac allograft rejection through NFкB-mediated inflammation in mouse heart transplantation.; Authors - Liuyi Lu
human,rat	WB,IHC	Brain Res; Dysfunctional microRNA-144-3p/ZBTB20/ERK/CREB1 signalling pathway is associated with MK-801-induced schizophrenia-like abnormalities; Authors - Bo Pan