YY2 Monoklonaler Antikörper

YY2 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

1C11A7

Kat-Nr. : 66839-1-Ig

Synonyme

Transcription factor YY2, Yin and yang 2, YY 2, YY2, YY2 transcription factor, Zinc finger protein 631, ZNF631

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Galerie der Validierungsdaten

• 

  WB analysis of human kidney using 66839-1-Ig

  human kidney tissue were subjected to SDS PAGE followed by western blot with 66839-1-Ig (YY2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 66839-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66839-1-Ig (YY2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 66839-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66839-1-Ig (YY2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Nierengewebe, humanes Plazenta-Gewebe, MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

66839-1-Ig bindet in WB, ELISA YY2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: YY2 fusion protein Ag25373
• Vollständiger Name: YY2 transcription factor
• Berechnetes Molekulargewicht: 372 aa, 46 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: BC137217
• Gene symbol: YY2
• Gene ID (NCBI): 404281
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

YY2 (Yin-Yang 2) encodes a zinc finger transcription factor similarity to the well-characterized YY1 that locating to nucleus. Both proteins recognize an identical DNA-binding motifs with a high homologous Zinc Finger region, and these transcription factors can act as activators or repressors when bound to DNA (PMID:26350623). It is reported that YY2 is placental mammal-specific and is absent in marsupial and non-mammalian vertebrate species (PMID:16377127). YYs are play an important role in multiple developmental processes , such as implantation, cell differentiation, X inactivation, imprinting and organogenesis (PMID:27191592). YY2 is expressed in kindey, liver, spleen and testicle but not in the colon (PMID:15087442). A 41kDa band could be detected.

Publikationen

Species	Application	Title
human	WB	Cell Death Dis; REV1 promotes lung tumorigenesis by activating the Rad18/SERTAD2 axis.; Authors - Yunshang Chen