YY1AP1 Polyklonaler Antikörper

YY1AP1 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 10425-1-AP

Synonyme

YY1AP, YY1 associated protein 1, YAP, Hepatocellular carcinoma-associated protein 2, HCCA2

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Galerie der Validierungsdaten

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  WB analysis of MDA-MB-231 using 10425-1-AP

  MDA-MB-231 cells were subjected to SDS PAGE followed by western blot with 10425-1-AP (YY1AP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 10425-1-AP

  Immunofluorescent analysis of HepG2 cells, using YY1AP1 antibody 10425-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MDA-MB-231-Zellen
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 2 publications below

Produktinformation

10425-1-AP bindet in WB, IF/ICC, ELISA YY1AP1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: YY1AP1 fusion protein Ag0692
• Vollständiger Name: YY1 associated protein 1
• Berechnetes Molekulargewicht: 75 kDa
• Beobachtetes Molekulargewicht: 75 kDa and 50 kDa
• GenBank-Zugangsnummer: BC003500
• Gene symbol: YY1AP1
• Gene ID (NCBI): 55249
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

YY1AP1 (YY1 associated protein 1), also known as HCCA2 (hepatocellular carcinoma-associated protein 2) or YY1AP, is a 796 amino acid protein that localizes to both the nucleus and the cytoplasm and exists as seven alternatively spliced isoforms. We detected 75 kDa and 50 kDa two isoforms. YY1AP1 is expressed ubiquitously with notable expression in lung, brain, spleen, pancreas, small intestine, heart, colon and skeletal muscle, YY1AP1 interacts with and enhances the transcriptional activation activity of YY1. YY1AP1 is expressed at high levels in liver carcinomas, suggesting a role in tumor transformation and metastasis. YY1AP1 may play a role in cell cycle regulation.

Publikationen

Species	Application	Title
human	WB,IF	Cell Death Differ; Inhibition of Drp1 SUMOylation by ALR protects the liver from ischemia-reperfusion injury.; Authors - Jing Huang
mouse	WB,IF	Psychol Aging; Cognitive engagement and cognitive aging: is openness protective; Authors - Emily Schoenhofen Sharp
mouse	WB	Eur J Pharmacol; Cannabidiol represses miR-143 to promote cardiomyocyte proliferation and heart regeneration after myocardial infarction; Authors - Zhongyu Ren