YTHDF2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

YTHDF2 Polyklonal Antikörper für IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (3)

Anwendung

WB, RIP, IP, IHC, IF, FC, CoIP, CLIP, chIP, ELISA

Konjugation

Unkonjugiert

Publikationen

235

Rezensionen (2)

Kat-Nr. : 24744-1-AP

Synonyme

CLL associated antigen KW 14, HGRG8, NY REN 2, YTH domain family protein 2, YTH domain family, member 2, YTHDF2

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Galerie der Validierungsdaten

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  WB analysis using 24744-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24744-1-AP (YTHDF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 24744-1-AP

  WB result of YTHDF2 antibody (24744-1-AP, 1:4000) with si-control and si-YTHDF2 transfected Jurkat cells..

• 

  WB analysis using 24744-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24744-1-AP (YTHDF2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 24744-1-AP

  IP result of anti-YTHDF2(IP:24744-1-AP, 4ug; Detection:24744-1-AP 1:6000) with HeLa cells lysate 1360 ug.

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  IF Staining of mouse brain using 24744-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 24744-1-AP (YTHDF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of U2OS using 24744-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using YTHDF2 antibody (24744-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of U2OS using 24744-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using YTHDF2 antibody (24744-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 24744-1-AP

  Immunofluorescent analysis of HeLa cells using 24744-1-AP (YTHDF2 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, C6-Zellen, HeLa-Zellen, Jurkat-Zellen, MOLT-4-Zellen, NIH/3T3-Zellen, Raji-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IF: Maushirngewebe, HeLa-Zellen, U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 72 publications below
• WB: See 189 publications below
• IHC: See 15 publications below
• IF: See 32 publications below
• IP: See 11 publications below
• FC: See 1 publications below
• CoIP: See 3 publications below
• ChIP: See 1 publications below
• RIP: See 50 publications below

Produktinformation

24744-1-AP bindet in WB, RIP, IP, IHC, IF, FC, CoIP, CLIP, chIP, ELISA YTHDF2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Hausschwein, Huhn, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: YTHDF2 fusion protein Ag5922
• Vollständiger Name: YTH domain family, member 2
• Berechnetes Molekulargewicht: 62 kDa
• Beobachtetes Molekulargewicht: 62 kDa
• GenBank-Zugangsnummer: BC002559
• Gene symbol: YTHDF2
• Gene ID (NCBI): 51441
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

YTHDF2 also named as CLL associated antigen KW 14 or HGRG8 is a 579 amino acid protein, which contains one YTH domain and exists as two alternatively spliced isoforms. YTHDF2 is expressed in in pancreas, testis and placenta. YTHDF2 may play a role in human longevity.

Publikationen

Species	Application	Title
human	IF	Nature; m6A enhances the phase separation potential of mRNA.; Authors - Ryan J Ries
mouse	RIP	Nature; m6A RNA methylation regulates the fate of endogenous retroviruses.; Authors - Tomasz Chelmicki ; KO Validated
mouse	WB	Nature; m6A facilitates hippocampus-dependent learning and memory through YTHDF1.; Authors - Hailing Shi
human, mouse	WB, IF	Nature; Dynamic m(6)A mRNA methylation directs translational control of heat shock response.; Authors - Jun Zhou
human	WB	Cell; G3BP1 Is a Tunable Switch that Triggers Phase Separation to Assemble Stress Granules.; Authors - Peiguo Yang
human,mouse	FC	Nat Med; Stem cell architecture drives myelodysplastic syndrome progression and predicts response to venetoclax-based therapy.; Authors - Irene Ganan-Gomez

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Jinyun (Verified Customer) (08-26-2022)

I did the western blot, and the antibody worked quite well.

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000

FH

Robert (Verified Customer) (01-24-2019)

1. scrambled siRNA2. YTHDF2 siRNA

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: ST cells (porcine)

YTHDF2 Antibody Western Blot, validation (1/1000 dilution) in ST cells (porcine) (Cat no:24744-1-AP)

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