MSY2 Polyklonaler Antikörper

MSY2 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, IHC, chIP, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 13538-1-AP

Synonyme

CONTRIN, CSDA3, DBPC, DNA binding protein C, MSY2, MSY2 homolog, Y box binding protein 2, YBX2

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Galerie der Validierungsdaten

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  WB analysis using 13538-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13538-1-AP (MSY2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 13538-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 13538-1-AP (MSY2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human stomach using 13538-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 13538-1-AP (MSY2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of PC-3 using 13538-1-AP

  IP result of anti-MSY2 (IP:13538-1-AP, 4ug; Detection:13538-1-AP 1:300) with PC-3 cells lysate 1720ug.

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  IHC staining of human ovary tumor using 13538-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 13538-1-AP (MSY2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary tumor using 13538-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 13538-1-AP (MSY2 antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, humanes Hodengewebe, humanes Magengewebe
• Erfolgreiche IP: PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 2 publications below
• ChIP: See 1 publications below

Produktinformation

13538-1-AP bindet in WB, IP, IF, IHC, chIP, ELISA MSY2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MSY2 fusion protein Ag4465
• Vollständiger Name: Y box binding protein 2
• Berechnetes Molekulargewicht: 364 aa, 39 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC033800
• Gene symbol: YBX2
• Gene ID (NCBI): 51087
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MSY2, also named as CSDA3 and YBX2, belongs to the Y box family of cold shock domain proteins. MSY2 is solely expressed in male and female germ cells and necessary for fertility. MSY2 serves as a coactivator of transcription, binding to the DNA sequence CTGATTGGC/TC/TAA in the promoters of genes transcribed in germ cells, thereby linking transcription with mRNA storage/stabilization (PMID: 19597149). MSY2 has 1 isoform with the molecular mass of 38 kDa. MSY2 is essential for regulating mRNA stability in oocytes (PMID: 21613634).

Publikationen

Species	Application	Title
mouse	WB,IHC,chIP	J Clin Invest; Methylation in pericytes after acute injury promotes chronic kidney disease.; Authors - Yu-Hsiang Chou
mouse	WB,IF,IP	Development; MFN2 interacts with nuage-associated proteins and is essential for male germ cell development by controlling mRNA fate during spermatogenesis; Authors -
mouse	WB,IF,IP	Development; MFN2 interacts with nuage-associated proteins and is essential for male germ cell development by controlling mRNA fate during spermatogenesis.; Authors - Xiaoli Wang