XRN1 Polyklonaler Antikörper

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• KD/KO Validated

XRN1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 23108-1-AP

Synonyme

5 3 exoribonuclease 1, 5' 3' exoribonuclease 1, SEP1, XRN1

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 23108-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23108-1-AP (XRN1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 23108-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain slide using 23108-1-AP (XRN1 Antibody) at dilution of 1:50.

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  IHC staining of human heart using 23108-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 23108-1-AP (XRN1 Antibody) at dilution of 1:50.

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  IHC staining of human brain using 23108-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain slide using 23108-1-AP (XRN1 Antibody) at dilution of 1:50.

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  IHC staining of human heart using 23108-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 23108-1-AP (XRN1 Antibody) at dilution of 1:50.

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  IF Staining of HeLa using 23108-1-AP

  Immunofluorescent analysis of HeLa cells using 23108-1-AP (XRN1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below

Produktinformation

23108-1-AP bindet in WB, IHC, IF, ELISA XRN1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: XRN1 fusion protein Ag19398
• Vollständiger Name: 5'-3' exoribonuclease 1
• Berechnetes Molekulargewicht: 1706 aa, 194 kDa
• Beobachtetes Molekulargewicht: 175 kDa
• GenBank-Zugangsnummer: BC078146
• Gene symbol: XRN1
• Gene ID (NCBI): 54464
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Exoribonuclease I (XRN1), also known as Sep1 or Rar5, is a 1,694-amino acid protein that functions as the major cytoplasmic 5 prime to 3 prime exoribonuclease and plays an important role in mRNA turnover. XRN1 may also function in the microtubular cytoskeleton as well as in DNA recombination and replication. XRN1 induces the expression of stress granules (SGs), cytoplasmic aggregates of stalled translational preinitiation complexes that accumulate during stress, and GW bodies/processing bodies (PBs), distinct cytoplasmic sites of mRNA degradation. There are several isoforms of XRN1 that are produced as a result of alternative splicing events.Loss of XRN1 markedly affects cell growth rates.This antibody is specific to the XRN1 protein.

Publikationen

Species	Application	Title
human	WB	Cancer Res; NAT10 drives cisplatin chemoresistance by enhancing ac4C-associated DNA repair in bladder cancer; Authors - Ruihui Xie
human	WB	Nucleic Acids Res; LINE-1 mRNA 3' end dynamics shape its biology and retrotransposition potential; Authors - Damian M Janecki ; KD Validated