WDR7 Polyklonaler Antikörper

WDR7 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 24431-1-AP

Synonyme

KIAA0541, Rabconnectin 3 beta, TRAG, WD repeat containing protein 7, WD repeat domain 7, WDR7

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  WB analysis using 24431-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24431-1-AP (WDR7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 24431-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24431-1-AP (WDR7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 24431-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24431-1-AP (WDR7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 24431-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24431-1-AP (WDR7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 24431-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24431-1-AP (WDR7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

24431-1-AP bindet in WB, IF, IHC, ELISA WDR7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: WDR7 fusion protein Ag19072
• Vollständiger Name: WD repeat domain 7
• Berechnetes Molekulargewicht: 1490 aa, 164 kDa
• Beobachtetes Molekulargewicht: 145-150 kDa
• GenBank-Zugangsnummer: BC131558
• Gene symbol: WDR7
• Gene ID (NCBI): 23335
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

WDR7, also named as KIAA0541 and TRAG, belongs to the WD-repeat protein family. WD repeats are areas with the repetitions of approximately 40 amino acids that have ends with trp-asp and gly-his amino acids (GH-WD) which may help to form a multi-protein complex. WDR7 is the gene encoding adjacent of lincRNA-RoR, which has an important role in apoptosis and cell cycle (PMID: 32128092). WDR7 has 2 isoforms with the molecular mass of 160 and 164 kDa. Sometimes lower molecular weight around 150 kDa can also be observed, which may be a modified variant of WDR7.

Publikationen

Species	Application	Title
	IF	J Biol Chem; The priming factor CAPS1 regulates dense-core vesicle acidification by interacting with rabconnectin3β/WDR7 in neuroendocrine cells.; Authors - Ellen Crummy