Vimentin Monoklonaler Antikörper

Vimentin Monoklonal Antikörper für IHC

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IHC

Konjugation

Biotin

CloneNo.

3H9D1

Kat-Nr. : Biotin-60330

Synonyme

Cell and organelle markers, Cytoskeleton Marker, FLJ36605, VIM, vimentin

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Galerie der Validierungsdaten

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  IHC staining of human tonsillitis using Biotin-60330

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using Biotin-60330 (Vimentin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using Biotin-60330

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using Biotin-60330 (Vimentin antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using Biotin-60330

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using Biotin-60330 (Vimentin antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using Biotin-60330

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using Biotin-60330 (Vimentin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using Biotin-60330

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using Biotin-60330 (Vimentin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

Biotin-60330 bindet in IHC Vimentin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Vimentin fusion protein Ag0489
• Vollständiger Name: vimentin
• Berechnetes Molekulargewicht: 466 aa, 54 kDa
• GenBank-Zugangsnummer: BC000163
• Gene symbol: VIM
• Gene ID (NCBI): 7431
• Konjugation: Biotin
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. And this antibody is conjugated with biotin.