VPS72 Polyklonaler Antikörper

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• KD/KO Validated

VPS72 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 15143-1-AP

Synonyme

CFL1, Protein YL 1, Swc2, TCFL1, Transcription factor like 1, VPS72, YL 1, YL1

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 15143-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15143-1-AP (VPS72 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 15143-1-AP

  WB result of VPS72 antibody (15143-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VPS72 transfected A549 cells.

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  WB analysis of mouse skeletal muscle using 15143-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15143-1-AP (VPS72 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 15143-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15143-1-AP (VPS72 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of NIH/3T3 using 15143-1-AP

  IP result of anti-VPS72 (IP:15143-1-AP, 4ug; Detection:15143-1-AP 1:500) with NIH/3T3 cells lysate 4000 ug.

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  IHC staining of human breast hyperplasia using 15143-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 15143-1-AP (VPS72 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast hyperplasia using 15143-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 15143-1-AP (VPS72 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast hyperplasia using 15143-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 15143-1-AP (VPS72 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast hyperplasia using 15143-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 15143-1-AP (VPS72 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, Maus-Skelettmuskelgewebe, NIH/3T3-Zellen
• Erfolgreiche IP: NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammahyperplasie-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

15143-1-AP bindet in WB, IP, IHC, ELISA VPS72 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VPS72 fusion protein Ag7424
• Vollständiger Name: vacuolar protein sorting 72 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 41 kDa
• Beobachtetes Molekulargewicht: 57 kDa
• GenBank-Zugangsnummer: BC003151
• Gene symbol: VPS72
• Gene ID (NCBI): 6944
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The mammalian TRRAP/TIP60-containing histone acetyltransferase (HAT) complex, which exists in Drosophila melanogaster and mammalian cells, is a complex that is responsible for various cellular processes, including DNA repair, transcriptional activation and apoptosis. Vacuolar protein sorting-associated protein 72 homolog (VPS72), also known as VPS72 or Transcription factor-like 1, is a 364 amino acid subunit of the TRRAP/TIP60 HAT complex. VPS72 has also been identified as a subunit of a novel complex containing SNF2-related helicase SRCAP (SWI2/SNF2-related CBP activator protein). This SRCAP-containing complex is very similar to the S. cerevisiae SWR1 chromatin remodeling complex. The involvement of VPS72 in these complexes has suggested that VPS72 plays multiple roles in chromatin modification and remodeling in cells. VPS72 localizes to the nucleus and is phosphorylated upon DNA damage, most likely by ATM or ATR.

Publikationen

Species	Application	Title
		Mol Cell; PMID: 28366643; Authors - Sophie Barral