VPS41 Polyklonaler Antikörper

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• KD/KO Validated

VPS41 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 13869-1-AP

Synonyme

HVPS41, hVps41p, HVSP41, S53, VPS41

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Galerie der Validierungsdaten

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  WB analysis of RAW 264.7 using 13869-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 13869-1-AP (VPS41 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 13869-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13869-1-AP (VPS41 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 13869-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13869-1-AP (VPS41 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 13869-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13869-1-AP (VPS41 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 13869-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13869-1-AP (VPS41 Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: RAW 264.7-Zellen, HepG2-Zellen, humanes Hirngewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 6 publications below
• CoIP: See 1 publications below

Produktinformation

13869-1-AP bindet in WB, IHC, CoIP, ELISA VPS41 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VPS41 fusion protein Ag4760
• Vollständiger Name: vacuolar protein sorting 41 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 854 aa, 98 kDa
• Beobachtetes Molekulargewicht: 94-100 kDa
• GenBank-Zugangsnummer: BC044851
• Gene symbol: VPS41
• Gene ID (NCBI): 27072
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. VPS41 (vacuolar protein sorting 41) is the human ortholog of yeast Vps41 protein. Yeast Vps41 is a subunit of AP3 (heterotetrameric adaptor protein complex 3) and HOPS (homotypic fusion and protein transport) complexes and is involved in trafficking of proteins from the late Golgi to the vacuole. In mammalian cells, Vps41 was found to be a member of HOPS complex, mediating the trafficking of proteins to lysosomes (PMID: 23173123). It has been reported that human VPS41 has a HOPS-independent function in the LAMP carrier pathway and also participates in lysosomal maturation, probably as part of the HOPS complex (PMID: 23322049).

Publikationen

Species	Application	Title
human	WB	Nat Commun; C9orf72-catalyzed GTP loading of Rab39A enables HOPS-mediated membrane tethering and fusion in mammalian autophagy; Authors - Shen Zhang
human	WB	Autophagy; SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes; Authors - Wang Zheng
human	WB,CoIP	Dev Cell; ORF3a of the COVID-19 virus SARS-CoV-2 blocks HOPS complex-mediated assembly of the SNARE complex required for autolysosome formation.; Authors - Guangyan Miao ; KD Validated
human	WB	J Cell Sci; Late endosomal transport and tethering are coupled processes controlled by RILP and the cholesterol sensor ORP1L.; Authors - van der Kant Rik R
human	WB	J Biol Chem; Characterization of the mammalian CORVET and HOPS complexes and their modular restructuring for endosome specificity.; Authors - Rik van der Kant
human	WB	J Cell Sci; The topology of lymphotoxin β receptor accumulated upon endolysosomal dysfunction dictates the NF-κB signaling outcome.; Authors - Magdalena Banach-Orłowska ; KD Validated