VEGFR2 Monoklonaler Antikörper

VEGFR2 Monoklonal Antikörper für FC, WB,ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

CloneNo.

1G2A8

Kat-Nr. : 67407-1-Ig

Synonyme

CD309, Fetal liver kinase 1, FLK 1, FLK1, KDR, Kinase insert domain receptor, VEGFR, VEGFR 2, VEGFR2

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Galerie der Validierungsdaten

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  WB analysis of human placenta using 67407-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67407-1-Ig (VEGFR2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  FC experiment of HUVEC using 67407-1-Ig

  1X10^6 HUVEC cells were intracellularly stained with 0.20 ug/test Anti-Human VEGFR2 (67407-1-Ig, Clone:1G2A8) (red) or 0.20 ug Mouse IgG1 Isotype Control(66360-1-Ig, CloneT1F8D3F10) (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000. Fixed with90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plazenta-Gewebe
• Erfolgreiche Detektion in FC: HUVEC-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Durchflusszytometrie (FC): FC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

67407-1-Ig bindet in WB, IHC, IF, FC, ELISA VEGFR2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: VEGFR2 fusion protein Ag24589
• Vollständiger Name: kinase insert domain receptor (a type III receptor tyrosine kinase)
• Berechnetes Molekulargewicht: 1356 aa, 152 kDa
• Beobachtetes Molekulargewicht: 230 kDa
• GenBank-Zugangsnummer: BC131822
• Gene symbol: KDR
• Gene ID (NCBI): 3791
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KDR, also named as VEGFR-2, FLK1 and CD309, is a receptor for VEGF or VEGFC. KDR which belongs to the protein kinase superfamily, has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. KDR functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. Mutations of this gene are implicated in infantile capillary hemangiomas.

Publikationen

Species	Application	Title
human	WB	Front Oncol; An Essential NRP1-Mediated Role for Tagln2 in Gastric Cancer Angiogenesis.; Authors - Hongwei Jin
human	WB	Front Genet; ANLN and KDR Are Jointly Prognostic of Breast Cancer Survival and Can Be Modulated for Triple Negative Breast Cancer Control.; Authors - Xiaofeng Dai
human	IHC,IF	Cell Tissue Res; A histological study of vascular wall resident stem cells in venous malformations; Authors - Hou-Fu Xia
human	WB	Phytother Res; Sclareol attenuates liver fibrosis through SENP1-mediated VEGFR2 SUMOylation and inhibition of downstream STAT3 signaling; Authors - Mao-Xu Ge
human	WB	Cell Signal; Exosomal B7-H3 facilitates colorectal cancer angiogenesis and metastasis through AKT1/mTOR/VEGFA pathway; Authors - Rendi Wu
human	WB	Cells; PLAC8-Mediated Activation of NOX4 Signalling Restores Angiogenic Function of Endothelial Colony-Forming Cells in Experimental Hypoxia; Authors - Shun Hay Pun