VASP Monoklonaler Antikörper
VASP Monoklonal Antikörper für WB, ELISA
Wirt / Isotyp
Maus / IgG2a
Getestete Reaktivität
human
Anwendung
WB, ELISA
Konjugation
Unkonjugiert
CloneNo.
1B9A11
Kat-Nr. : 68431-1-Ig
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | U2OS-Zellen, A431-Zellen, A549-Zellen, HeLa-Zellen, K-562-Zellen, MCF-7-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
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Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Produktinformation
68431-1-Ig bindet in WB, ELISA VASP und zeigt Reaktivität mit human
Getestete Reaktivität | human |
Wirt / Isotyp | Maus / IgG2a |
Klonalität | Monoklonal |
Typ | Antikörper |
Immunogen | VASP fusion protein Ag17679 |
Vollständiger Name | vasodilator-stimulated phosphoprotein |
Berechnetes Molekulargewicht | 380 aa, 40 kDa |
Beobachtetes Molekulargewicht | 46 kDa |
GenBank-Zugangsnummer | BC038224 |
Gene symbol | VASP |
Gene ID (NCBI) | 7408 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Protein-A-Reinigung |
Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
VASP belongs to the Ena/VASP family. Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. VASP plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation. Human platelet activation is inhibited by agents such as prostaglandins and NO donors, which elevate cAMP or cGMP levels. VASP is phosphorylated in human platelets in response to both cAMP- and cGMP-elevating agents, and its phosphorylation correlates with platelet inhibition. VASP is located about 92 kb distal to ERCC1 (126380) and about 300 kb proximal to the myotonic dystrophy protein kinase gene. The antibody is specific to VASP.
Protokolle
Produktspezifische Protokolle | |
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WB protocol for VASP antibody 68431-1-Ig | Protokoll herunterladen |
Standard-Protokolle | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |