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VASP Polyklonaler Antikörper

VASP Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13472-1-AP

Synonyme

Vasodilator-stimulated phosphoprotein



Geprüfte Anwendungen

Erfolgreiche Detektion in WBA549-Zellen, C6-Zellen, HEK-293-Zellen, HeLa-Zellen, Mausmilzgewebe
Erfolgreiche IPHEK-293-Zellen
Erfolgreiche Detektion in IHChumanes Hodengewebe, humanes Hautkrebsgewebe, humanes Herzgewebe, humanes Lungengewebe, humanes Milzgewebe, humanes Nierengewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCHepG2-Zellen
Erfolgreiche Detektion in FC (Intra)HepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:6000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:20-1:200
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

13472-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA VASP und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen VASP fusion protein Ag4266
Vollständiger Name vasodilator-stimulated phosphoprotein
Berechnetes Molekulargewicht 380 aa, 40 kDa
Beobachtetes Molekulargewicht 46 kDa, 50 kDa
GenBank-ZugangsnummerBC038224
Gene symbol VASP
Gene ID (NCBI) 7408
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VASP belongs to the Ena/VASP family. Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. VASP plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation. Human platelet activation is inhibited by agents such as prostaglandins and NO donors, which elevate cAMP or cGMP levels. VASP is phosphorylated in human platelets in response to both cAMP- and cGMP-elevating agents, and its phosphorylation correlates with platelet inhibition. VASP is located about 92 kb distal to ERCC1 (126380) and about 300 kb proximal to the myotonic dystrophy protein kinase gene. The antibody is specific to VASP.

Protokolle

Produktspezifische Protokolle
WB protocol for VASP antibody 13472-1-APProtokoll herunterladen
IHC protocol for VASP antibody 13472-1-APProtokoll herunterladen
IF protocol for VASP antibody 13472-1-APProtokoll herunterladen
IP protocol for VASP antibody 13472-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanIF

Nat Commun

Cortical dynamics during cell motility are regulated by CRL3(KLHL21) E3 ubiquitin ligase.

Authors - Thibault Courtheoux
humanWB

PLoS Biol

Nucleotide de novo synthesis increases breast cancer stemness and metastasis via cGMP-PKG-MAPK signaling pathway.

Authors - Yajing Lv
humanWB, IF, IHC

Oncogene

The Wnt/β-catenin/VASP positive feedback loop drives cell proliferation and migration in breast cancer.

Authors - Kai Li
  • KD Validated
humanWB

Front Oncol

High-Risk HPV16 E6 Activates the cGMP/PKG Pathway Through Glycosyltransferase ST6GAL1 in Cervical Cancer Cells.

Authors - Jun Wang
humanWB

Oncol Rep

TNF‑α inhibits xenograft tumor formation by A549 lung cancer cells in nude mice via the HIF‑1α/VASP signaling pathway.

Authors - Wen Liu
  • KD Validated
humanWB

Pathol Res Pract

Silencing lncRNA SNHG6 suppresses proliferation and invasion of breast cancer cells through miR-26a/VASP axis.

Authors - Kai Li