VASH1 Polyklonaler Antikörper

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• KD/KO Validated

VASH1 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 12730-1-AP

Synonyme

Tyrosine carboxypeptidase 1, Tubulinyl-Tyr carboxypeptidase 1, Tubulin carboxypeptidase 1, TTCP 1, KIAA1036

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Galerie der Validierungsdaten

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  WB analysis of human brain using 12730-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12730-1-AP (VASH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 12730-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 12730-1-AP (VASH1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12730-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12730-1-AP (VASH1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 12730-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using VASH1 antibody (12730-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, HeLa-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 2 publications below

Produktinformation

12730-1-AP bindet in WB, IF/ICC, ELISA VASH1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VASH1 fusion protein Ag3435
• Vollständiger Name: vasohibin 1
• Berechnetes Molekulargewicht: 365 aa, 41 kDa
• Beobachtetes Molekulargewicht: 36 and 41 kDa
• GenBank-Zugangsnummer: BC009031
• Gene symbol: VASH1
• Gene ID (NCBI): 22846
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VASH1, also named as vasohibin 1, is a angiogenesis inhibitor induced by the stimulation with VEGF. High expression of VASH1 has been researched in some tumors as a result of VEGF induction. It has been suggested that VASH-1 is clinically significant in bladder cancer, colorectal cancer and NSCLC. VASH-1 may be a potential marker of gastric cancer and a target for vascular targeting therapy. 12730-1-AP detects 41 kDa form secreted protein and 36 kDa form protein accumulated within the cells or pericellular milieu in SDS-PAGE.(PMID:19057892, 29042936, 29552154, 26666821)

Publikationen

Species	Application	Title
zebrafish	WB	Development; Vasohibin 1 selectively regulates secondary sprouting and lymphangiogenesis in the zebrafish trunk.; Authors - Marta Bastos de Oliveira ; KO Validated
	WB	Cell Rep; Augmin-dependent microtubule self-organization drives kinetochore fiber maturation in mammals.; Authors - Ana C Almeida ; KD Validated