VAMP5 Polyklonaler Antikörper

VAMP5 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Rezensionen (1)

Kat-Nr. : 11822-1-AP

Synonyme

Myobrevin, VAMP 5, VAMP5

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Galerie der Validierungsdaten

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  WB analysis of human lung using 11822-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 11822-1-AP (VAMP5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human peripheral blood leukocyte using 11822-1-AP

  Human peripheral blood leukocyte cells were subjected to SDS PAGE followed by western blot with 11822-1-AP (VAMP5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 11822-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11822-1-AP (VAMP5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 11822-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11822-1-AP (VAMP5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 11822-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11822-1-AP (VAMP5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Lungengewebe, humane Leukozytenzellen aus peripherem Blut, Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

11822-1-AP bindet in WB, IHC, ELISA VAMP5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VAMP5 fusion protein Ag2390
• Vollständiger Name: vesicle-associated membrane protein 5 (myobrevin)
• Berechnetes Molekulargewicht: 12.8 kDa
• Beobachtetes Molekulargewicht: 13-15 kDa
• GenBank-Zugangsnummer: BC017891
• Gene symbol: VAMP5
• Gene ID (NCBI): 10791
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VAMP5, also known as myobrevin, is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family and the SNARE superfamily. Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP5 may participate in trafficking events that are associated with myogenesis, such as myoblast fusion and/or GLUT4 trafficking.

Publikationen

Species	Application	Title
human	WB,IHC	Front Immunol; Immune Gene Signatures and Immunotypes in Immune Microenvironment Are Associated With Glioma Prognose.; Authors - Xiang-Xu Wang
human	WB	Front Mol Neurosci; Construction and validation of a risk model based on the key SNARE proteins to predict the prognosis and immune microenvironment of gliomas; Authors - Luxin Yin

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Boyan (Verified Customer) (06-19-2020)

This antibody labels a ~ 25 kD band, but not a 15 kD band, in 293T cell lysate - not sure is this a nonspecific band or a special version of this protein in 293T cells.

• Applications: Western Blot