USP25 Polyklonaler Antikörper

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• KD/KO Validated

USP25 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 12199-1-AP

Synonyme

Deubiquitinating enzyme 25, Ubiquitin thiolesterase 25, USP on chromosome 21, USP21, USP25

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Galerie der Validierungsdaten

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  WB analysis using 12199-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12199-1-AP (USP25 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse cerebellum using 12199-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 12199-1-AP (USP25 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of RAW 264.7 using 12199-1-AP

  IP result of anti-USP25 (IP:12199-1-AP, 4ug; Detection:12199-1-AP 1:300) with RAW 264.7 cells lysate 1600ug.

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  IHC staining of human lung cancer using 12199-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12199-1-AP (USP25 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 12199-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12199-1-AP (USP25 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: BxPC-3-Zellen, K-562-Zellen, Maus-Cerebellum-Gewebe
• Erfolgreiche IP: RAW 264.7-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 1 publications below

Produktinformation

12199-1-AP bindet in WB, IP, IHC, ELISA USP25 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: USP25 fusion protein Ag2837
• Vollständiger Name: ubiquitin specific peptidase 25
• Berechnetes Molekulargewicht: 125 kDa
• Beobachtetes Molekulargewicht: 125 kDa
• GenBank-Zugangsnummer: BC015930
• Gene symbol: USP25
• Gene ID (NCBI): 29761
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ubiquitin carboxyl-terminal hydrolase 25, encoded by USP25 gene, has three isoforms by alternative splicing. Isoform USP25a is found in most adult and fetal tissues; Isoform USP25b is found in all tissues except heart and skeletal muscle. Isoform USP25m is heart and skeletal muscle specific, and is upregulated during myogenesis, therefore, may have a role in the regulation of muscular differentiation and function. USP25, as deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates.

Publikationen

Species	Application	Title
human	WB	Mol Cell; An Interaction Landscape of Ubiquitin Signaling.; Authors - Xiaofei Zhang
human	WB	Cell Death Dis; Suppression of USP7 induces BCR-ABL degradation and chronic myelogenous leukemia cell apoptosis.; Authors - Shuoyi Jiang
mouse	WB	Biochem Pharmacol; Inhibition of deubiquitinase USP28 attenuates cyst growth in autosomal dominant polycystic kidney disease; Authors - Ying Ren
human	WB	Nucleic Acids Res; The dimeric deubiquitinase USP28 integrates 53BP1 and MYC functions to limit DNA damage; Authors - Chao Jin
mouse	WB	Ren Fail; USP25 attenuates anti-GBM nephritis in mice by negative feedback regulation of Th17 cell differentiation; Authors - Ranran Xu ; KO Validated
human	IHC	J Cell Physiol; The miR-27a-3p/USP25 axis participates in the pathogenesis of recurrent miscarriage by inhibiting trophoblast migration and invasion.; Authors - Jinli Ding ; KD Validated