USP24 Polyklonaler Antikörper

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• KD/KO Validated

USP24 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(18)

Kat-Nr. : 13126-1-AP

Synonyme

Deubiquitinating enzyme 24, EC:3.4.19.12, KIAA1057, Ubiquitin carboxyl-terminal hydrolase 24, Ubiquitin thioesterase 24

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  WB analysis of HepG2 using 13126-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13126-1-AP (USP24 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 13126-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13126-1-AP (USP24 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of rat lung using 13126-1-AP

  rat lung tissue were subjected to SDS PAGE followed by western blot with 13126-1-AP (USP24 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 13126-1-AP

  IP result of anti-USP24 (IP:13126-1-AP, 4ug; Detection:13126-1-AP 1:3000) with mouse brain tissue lysate 1680 ug.

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  IHC staining of human lymphoma using 13126-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13126-1-AP (USP24 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 13126-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13126-1-AP (USP24 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 13126-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using USP24 antibody (13126-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 13126-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using USP24 antibody (13126-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Mauslungengewebe, Rattenlungengewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 15 publications below
• IHC: See 5 publications below
• IF: See 3 publications below
• IP: See 2 publications below

Produktinformation

13126-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA USP24 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: USP24 fusion protein Ag3779
• Vollständiger Name: ubiquitin specific peptidase 24
• Berechnetes Molekulargewicht: 294 kDa
• Beobachtetes Molekulargewicht: 280-294 kDa
• GenBank-Zugangsnummer: BC029660
• Gene symbol: USP24
• Gene ID (NCBI): 23358
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Mol Cancer; LINE-1 promotes tumorigenicity and exacerbates tumor progression via stimulating metabolism reprogramming in non-small cell lung cancer; Authors - Zeguo Sun
human	WB,IHC,IF	Nat Commun; USP24 induces IL-6 in tumor-associated microenvironment by stabilizing p300 and β-TrCP and promotes cancer malignancy.; Authors - Yi-Chang Wang ; KD Validated
mouse	WB	Nat Commun; A RUNX2 stabilization pathway mediates physiologic and pathologic bone formation.; Authors - Jung-Min Kim ; KD Validated
mouse		Nat Commun; ERAP1 promotes Hedgehog-dependent tumorigenesis by controlling USP47-mediated degradation of βTrCP.; Authors - Francesca Bufalieri
human	WB,IP	Cell Death Differ; USP24 promotes drug resistance during cancer therapy.; Authors - Shao-An Wang
human	WB	EMBO Mol Med; USP9X stabilizes XIAP to regulate mitotic cell death and chemoresistance in aggressive B-cell lymphoma.; Authors - Katharina Engel