USP2 Polyklonaler Antikörper

USP2 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15404-1-AP

Synonyme

Deubiquitinating enzyme 2, ubiquitin specific peptidase 2, Ubiquitin thiolesterase 2, UBP41, USP2, USP9

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Galerie der Validierungsdaten

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  WB analysis of mouse testis using 15404-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 15404-1-AP (USP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 15404-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 15404-1-AP (USP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 15404-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15404-1-AP (USP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 15404-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15404-1-AP (USP2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 15404-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15404-1-AP (USP2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 15404-1-AP

  Immunofluorescent analysis of HepG2 cells, using USP2 antibody 15404-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, Maushirngewebe, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

15404-1-AP bindet in WB, IHC, IF, ELISA USP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: USP2 fusion protein Ag7658
• Vollständiger Name: ubiquitin specific peptidase 2
• Berechnetes Molekulargewicht: 68 kDa
• Beobachtetes Molekulargewicht: 41-50 kDa, 68 kDa
• GenBank-Zugangsnummer: BC002854
• Gene symbol: USP2
• Gene ID (NCBI): 9099
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ubiquitin, a highly conserved protein involved in the regulation of intracellular protein breakdown, cell cycle regulation, and stress response, is released from degraded proteins by disassembly of the polyubiquitin chains. The disassembly process is mediated by ubiquitin-specific proteases (USPs). USP2 is also named as UBP41. USP2 can interact indirectly with multiple clock proteins, so it remains possible that USP2 may modulate the ubiquitination and function of these other proteins. It has 4 isoforms produced by alternative splicing with the MW of 68, 41, 40 and 45 kDa. USP2 can exsit as a homooligomer.