USP2 Polyklonaler Antikörper

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• KD/KO Validated

USP2 Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

18

Kat-Nr. : 10392-1-AP

Synonyme

USP9, UBP41, Ubiquitin thiolesterase 2, ubiquitin specific peptidase 2, EC:3.4.19.12

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Galerie der Validierungsdaten

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  WB analysis using 10392-1-AP

  NIH/3T3 lysates were subjected to SDS PAGE followed by western blot with 10392-1-AP (USP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10392-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10392-1-AP (USP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 10392-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10392-1-AP (USP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 10392-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10392-1-AP (USP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 10392-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10392-1-AP (USP2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10392-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10392-1-AP (USP2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 10392-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10392-1-AP (USP2 antibody) at dilution of 1:100 (under 25x lens).

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  IHC staining of human skeletal muscle using 10392-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 10392-1-AP (USP2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 10392-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 10392-1-AP (USP2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 10392-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10392-1-AP (USP2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 10392-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10392-1-AP (USP2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of mouse embryo using 10392-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using 10392-1-AP (USP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse embryo using 10392-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using 10392-1-AP (USP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, HEK-293-Zellen, Maushirngewebe, Mausnierengewebe, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Prostatakarzinomgewebe, humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Mausembryogewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 15 publications below
• IHC: See 8 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Produktinformation

10392-1-AP bindet in WB, IHC, IF-P, CoIP, ELISA USP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: USP2 fusion protein Ag0591
• Vollständiger Name: ubiquitin specific peptidase 2
• Berechnetes Molekulargewicht: 41 kDa, 69 kDa
• Beobachtetes Molekulargewicht: 68 kDa, 41-45 kDa
• GenBank-Zugangsnummer: BC002955
• Gene symbol: USP2
• Gene ID (NCBI): 9099
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ubiquitin, a highly conserved protein involved in the regulation of intracellular protein breakdown, cell cycle regulation, and stress response, is released from degraded proteins by disassembly of the polyubiquitin chains. The disassembly process is mediated by ubiquitin-specific proteases (USPs). USP2 is also named as UBP41. USP2 can interact indirectly with multiple clock proteins, so it remains possible that USP2 may modulate the ubiquitination and function of these other proteins. It has 4 isoforms produced by alternative splicing. This antibody is specific to the isoform 1(USP2a) of USP2.

Publikationen

Species	Application	Title
human	WB,IHC	Gastroenterology; Dual inhibition of CDK4/6 and XPO1 induces senescence with acquired vulnerability to CRBN-based PROTAC drugs; Authors - Hui Wang
human	WB	FASEB J; Ubiquitin-specific protease 2-69 in macrophages potentially modulates metainflammation.; Authors - Kitamura Hiroshi H
rat	WB	J Cell Mol Med; Ubiquitin-specific protease 2 regulates Ang Ⅱ-induced cardiac fibroblasts activation by up-regulating cyclin D1 and stabilizing β-catenin in vitro.; Authors - Qiong Xu ; KD Validated
mouse	WB	Mol Nutr Food Res; 3,3'-Diindolylmethane suppresses high-fat diet-induced obesity through inhibiting adipogenesis of pre-adipocytes by targeting USP2 activity.; Authors - Hee Yang
mouse	WB,IHC	Mediators Inflamm; Overexpression of Ubiquitin-Specific Protease 2 (USP2) in the Heart Suppressed Pressure Overload-Induced Cardiac Remodeling.; Authors - Junhui Xing
human,mouse	WB,IF,CoIP	J Cancer Res Clin Oncol; USP2 promotes cell proliferation and metastasis in choroidal melanoma via stabilizing Snail; Authors - Chao Wei