URG4 Polyklonaler Antikörper

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• KD/KO Validated

URG4 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 11998-1-AP

Synonyme

URGCP, Up-regulator of cell proliferation, up regulated gene 4, KIAA1507, HBxAg up-regulated gene 4 protein

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 11998-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11998-1-AP (URG4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11998-1-AP

  WB result of URG4 antibody (11998-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-URG4 transfected HEK-293 cells.

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  WB analysis of HEK-293 using 11998-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11998-1-AP (URG4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 11998-1-AP

  IP result of anti-URG4 (IP:11998-1-AP, 4ug; Detection:11998-1-AP 1:300) with HEK-293 cells lysate 880ug.

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  IHC staining of human gliomas using 11998-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11998-1-AP (URG4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human stomach cancer using 11998-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11998-1-AP (URG4 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human stomach cancer using 11998-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11998-1-AP (URG4 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver cancer using 11998-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11998-1-AP (URG4 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 11998-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11998-1-AP (URG4 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 11998-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using URG4 antibody (11998-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe, humanes Leberkarzinomgewebe, humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

11998-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA URG4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: URG4 fusion protein Ag2630
• Vollständiger Name: up-regulated gene 4
• Berechnetes Molekulargewicht: 922 aa, 104 kDa
• Beobachtetes Molekulargewicht: 104 kDa
• GenBank-Zugangsnummer: BC018426
• Gene symbol: URG4
• Gene ID (NCBI): 55665
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

URG4 (upregulated gene 4), also known as upregulator of cell proliferation (URGCP), is an oncogene which is involved in the development and progression of various tumors. URG4 is upregulated in hepatocellular carcinoma (HCC), ovarian cancer, and other types of cancer. Overexpression of URG4 not only results in tumour progression but also in metastasis and recurrence.

Publikationen

Species	Application	Title
human	WB	Bone; Osteoblast-derived exosomes promote osteogenic differentiation of osteosarcoma cells via URG4/Wnt signaling pathway; Authors - Yuanxi Leng
human	IHC,WB	J Orthop Surg Res; URG4 mediates cell proliferation and cell cycle in osteosarcoma via GSK3β/β-catenin/cyclin D1 signaling pathway.; Authors - Yayun Liu ; KD Validated