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UNC119 Polyklonaler Antikörper

UNC119 Polyklonal Antikörper für IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Hund

Anwendung

IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13065-1-AP

Synonyme

unc 119 homolog (C. elegans), RG4, Retinal protein 4, Protein unc-119 homolog A, Protein unc 119 homolog A

Galerie der Validierungsdaten

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  • Immunofluorescence (IF) / fluorescent staining of MDCK cells using UNC119 Polyclonal antibody (13065-1-AP)

    IF Staining of MDCK using 13065-1-AP

    Immunofluorescent analysis of MDCK cells using 13065-1-AP (UNC119 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Geprüfte Anwendungen

Erfolgreiche Detektion in IF/ICCMDCK-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

IHCSee 1 publications below

Produktinformation

13065-1-AP bindet in IHC, IF/ICC, ELISA UNC119 und zeigt Reaktivität mit human, Hund

Getestete Reaktivität human, Hund
In Publikationen genannte Reaktivitäthuman
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen UNC119 fusion protein Ag3757
Vollständiger Name unc-119 homolog (C. elegans)
Berechnetes Molekulargewicht 240 aa, 27 kDa
GenBank-ZugangsnummerBC027176
Gene symbol UNC119
Gene ID (NCBI) 9094
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human UNC119 protein shares strong homology with the C. elegans unc119 protein which was first discovered in C. elegans on the basis of a spontaneous mutation affecting locomotion, feeding behavior and chemosensation. UNC119 is predominantly expressed in retina, in photoreceptor synapses and inner segments, with much lower expression in several other tissues. UNC119 is involved in the mechanism of photoreceptor neurotransmitter release through the synaptic vesicle cycle. It is required for G protein trafficking in sensory neurons.

Protokolle

Produktspezifische Protokolle
IF protocol for UNC119 antibody 13065-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanIHC

Int J Mol Sci

Ribbon Synapses and Retinal Disease: Review

Authors - Courtney E Frederick
View Article

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Francesc R (Verified Customer) (06-14-2022)

Antibody works nicely by Western blot, specifically recognizing band of roughly the expected size. Attached image shows blot of CRISPR clones: four still contain UNC119 and all the others without the band are the UNC119-KO clones.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293T
UNC119 Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:13065-1-AP)