UIMC1 Polyklonaler Antikörper

UIMC1 Polyklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13642-1-AP

Synonyme

BRCA1 A complex subunit RAP80, RAP80, Receptor associated protein 80, RXRIP110, UIMC1, X2HRIP110

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Galerie der Validierungsdaten

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  WB analysis of mouse colon using 13642-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 13642-1-AP (UIMC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13642-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13642-1-AP (UIMC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 13642-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 13642-1-AP (UIMC1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 13642-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 13642-1-AP (UIMC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 13642-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 13642-1-AP (UIMC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13642-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13642-1-AP (UIMC1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of COLO 320 using 13642-1-AP

  IP Result of anti-UIMC1 (IP:13642-1-AP, 4ug; Detection:13642-1-AP 1:500) with COLO 320 cells lysate 2400ug.

• 

  IHC staining of human testis using 13642-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13642-1-AP (UIMC1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 13642-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13642-1-AP (UIMC1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 13642-1-AP

  Immunofluorescent analysis of Hela cells, using UIMC1 antibody 13642-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  IF Staining of HeLa using 13642-1-AP

  Immunofluorescent analysis of Hela cells, using UIMC1 antibody 13642-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  IF Staining of MCF-7 using 13642-1-AP

  Immunofluorescent analysis of MCF-7 cells, using UIMC1 antibody 13642-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Kolongewebe, COLO 320-Zellen, HeLa-Zellen, Raji-Zellen
• Erfolgreiche IP: COLO 320-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen, MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

13642-1-AP bindet in WB, IP, IHC, IF,ELISA UIMC1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UIMC1 fusion protein Ag4582
• Vollständiger Name: ubiquitin interaction motif containing 1
• Berechnetes Molekulargewicht: 719 aa, 80 kDa
• Beobachtetes Molekulargewicht: 97 kDa, 80 kDa, 71-75 kDa, 61 kDa
• GenBank-Zugangsnummer: BC032561
• Gene symbol: UIMC1
• Gene ID (NCBI): 51720
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.