UGT8 Polyklonaler Antikörper

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• KD/KO Validated

UGT8 Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 17982-1-AP

Synonyme

ceramide galactosyltransferase, Cerebroside synthase, CGT, UDP glycosyltransferase 8, UGT4, UGT8

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Galerie der Validierungsdaten

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  WB analysis of rat brain using 17982-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 17982-1-AP (UGT8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 17982-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17982-1-AP (UGT8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse cerebellum using 17982-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 17982-1-AP (UGT8 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of rat brain using 17982-1-AP

  IP Result of anti-UGT8 (IP:17982-1-AP, 4ug; Detection:17982-1-AP 1:1000) with rat brain tissue lysate 4000ug.

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  IHC staining of human brain using 17982-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17982-1-AP (UGT8 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 17982-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17982-1-AP (UGT8 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human breast cancer using 17982-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17982-1-AP (UGT8 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 17982-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17982-1-AP (UGT8 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenhirngewebe, Maus-Cerebellum-Gewebe, Maushirngewebe
• Erfolgreiche IP: Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 5 publications below
• IF: See 1 publications below

Produktinformation

17982-1-AP bindet in WB, IP, IHC, IF, ELISA UGT8 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UGT8 fusion protein Ag12393
• Vollständiger Name: UDP glycosyltransferase 8
• Berechnetes Molekulargewicht: 541 aa, 61 kDa
• Beobachtetes Molekulargewicht: 61 kDa
• GenBank-Zugangsnummer: BC075069
• Gene symbol: UGT8
• Gene ID (NCBI): 7368
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UGT8, also known as galacotosylceramide synthase or CGT (ceramide galactosyltransferase), is a key enzyme for galacotosylceramide (GalCer) biosynthesis. It is an ER transmembrane protein and has limited tissue distribution, with predominance in Schwann cells, oligodendrocytes, kidneys, testes, and intestine. UGT8 is highly enriched in myelin in the central nervous system. Its expression levels are strongly associated with histological typing in human oligodendrogliomas and astrocytomas; can be used as molecular marker to distinguish these tumors. Higher UGT8 levels had also been linked to metastatic properties of cancer cells. This antibody specifically recognizes the endogenous UGT8.

Publikationen

Species	Application	Title
human	WB,IF	J Exp Med; Inhibition of UGT8 suppresses basal-like breast cancer progression by attenuating sulfatide-αVβ5 axis.; Authors - Qianhua Cao ; KD Validated
mouse,human	WB,IHC	Mol Cell Proteomics; Metabolic Enzyme Alterations and Astrocyte Dysfunction in a Murine Model of Alexander Disease With Severe Reactive Gliosis.; Authors - Michael R Heaven
human	WB	Mol Pharmacol; Circular RNAs of UDP-Glycosyltransferase (UGT) Genes Expand the Complexity and Diversity of the UGT Transcriptome.; Authors - Dong Gui Hu
human	IHC	Biosci Rep; Uronic acid metabolic process-related gene expression-based signature predicts overall survival of glioma.; Authors - Yuemei Feng
rat, human	WB,IHC	Cancer Chemother Pharmacol; Evaluation of resveratrol sensitivities and metabolic patterns in human and rat glioblastoma cells.; Authors - Sun Zheng Z
rat	WB, IHC	Neurotherapeutics; Diffusion Efficiency and Bioavailability of Resveratrol Administered to Rat Brain by Different Routes: Therapeutic Implications.; Authors - Xiao-Hong Shu