UGCG Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

UGCG Polyklonal Antikörper für WB, IHC, IF-P, IF-Fro, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, IF-Fro, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Rezensionen (1)

Kat-Nr. : 12869-1-AP

Synonyme

Glycosylceramide synthase, Glucosylceramide synthase, GLCT-1, GCS, EC:2.4.1.80

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Galerie der Validierungsdaten

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  WB analysis of A375 using 12869-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 12869-1-AP (UGCG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 12869-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 12869-1-AP (UGCG Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of U-251 using 12869-1-AP

  U-251 cells were subjected to SDS PAGE followed by western blot with 12869-1-AP (UGCG Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12869-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12869-1-AP (UGCG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A375 using 12869-1-AP

  IP result of anti-UGCG (IP:12869-1-AP, 4ug; Detection:12869-1-AP 1:500) with A375 cells lysate 3200ug.

• 

  IHC staining of human lung cancer using 12869-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12869-1-AP (UGCG Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12869-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12869-1-AP (UGCG Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 12869-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 12869-1-AP (UGCG antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human breast cancer using 12869-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12869-1-AP (UGCG Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12869-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12869-1-AP (UGCG Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse kidney using 12869-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using UGCG antibody (12869-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 12869-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using UGCG antibody (12869-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse kidney using 12869-1-AP

  Immunofluorescent analysis of frozen mouse kidney tissue fixed with 4% PFA for 24 hours, embedded in OCT, permeabilized with 0.5% Triton X-100 PBS and subjected to antigen retrieval in sodium citrate buffer (pH 6.0) using UGCG antibody (12869-1-AP) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, C6-Zellen, Maushirngewebe, U-251-Zellen
• Erfolgreiche IP: A375-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Lymphomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Mausnierengewebe, Maushirngewebe
• Erfolgreiche Detektion in IF-Fro: Mausnierengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)-FRO: IF-FRO : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 3 publications below
• IF: See 4 publications below

Produktinformation

12869-1-AP bindet in WB, IHC, IF-P, IF-Fro, IP, ELISA UGCG und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UGCG fusion protein Ag3530
• Vollständiger Name: UDP-glucose ceramide glucosyltransferase
• Berechnetes Molekulargewicht: 394 aa, 45 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC038711
• Gene symbol: UGCG
• Gene ID (NCBI): 7357
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UGCG, also named as GCS and GLCT-1, belongs to the glycosyltransferase 2 family. It catalyzes the first glycosylation step in glycosphingolipid biosynthesis, the transfer of glucose to ceramide. UGCG may also serve as a "flippase". It is up-regulated at the transcriptional level during keratinocyte differentiation. UGCG is a membrane protein. The calculated MW is 45 kDa. In SDS-PAGE, it is about 50-55 kDa.

Publikationen

Species	Application	Title
human	WB	Nat Commun; Glycolysis regulates KRAS plasma membrane localization and function through defined glycosphingolipids; Authors - Junchen Liu
human,mouse	WB	Cancer Res; Aneuploid cell survival relies upon sphingolipid homeostasis.; Authors - Yun-Chi Tang ; KD Validated
mouse	WB,IF	Cell Mol Biol Lett; UGCG modulates heart hypertrophy through B4GalT5-mediated mitochondrial oxidative stress and the ERK signaling pathway; Authors - Shengyu Cui
human, mouse	WB,IHC	Hum Mol Genet; Amyotrophic lateral sclerosis and denervation alter sphingolipids and up-regulate glucosylceramide synthase.; Authors - Alexandre Henriques
human	WB,IHC	Aging (Albany NY); Identification of long-term survival-associated gene in breast cancer.; Authors - Shipeng Ning
human	IF	iScience; Liver sphingomyelin synthase 1 deficiency causes steatosis, steatohepatitis, fibrosis, and tumorigenesis: An effect of glucosylceramide accumulation.; Authors - Zhiqiang Li

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Will (Verified Customer) (01-31-2019)

good antibody, would order again

• Applications: Western Blot,