UCK1 Polyklonaler Antikörper

UCK1 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Rezensionen (1)

Kat-Nr. : 12271-1-AP

Synonyme

Uridine-cytidine kinase 1, Uridine monophosphokinase 1, uridine cytidine kinase 1, UCK 1, EC:2.7.1.48

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Galerie der Validierungsdaten

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  WB analysis using 12271-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12271-1-AP (UCK1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 12271-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12271-1-AP (UCK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 12271-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12271-1-AP (UCK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 12271-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12271-1-AP (UCK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 12271-1-AP

  IP result of anti-UCK1 (IP:12271-1-AP, 4ug; Detection:12271-1-AP 1:1000) with MCF-7 cells lysate 2800ug.

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  IF Staining of MCF-7 using 12271-1-AP

  Immunofluorescent analysis of MCF-7 cells using 12271-1-AP (UCK1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, MCF-7-Zellen
• Erfolgreiche IP: MCF-7-Zellen
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

12271-1-AP bindet in WB, IF/ICC, IP, ELISA UCK1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UCK1 fusion protein Ag2912
• Vollständiger Name: uridine-cytidine kinase 1
• Berechnetes Molekulargewicht: 277 aa, 31 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: BC015547
• Gene symbol: UCK1
• Gene ID (NCBI): 83549
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Uridine/cytidine kinase-1 is a pyrimidine ribonucleoside kinase that catalyzes the phosphorylation of uridine and cytidine to form uridine monophosphate (UMP) and cytidine monophosphate (CMP). The deduced 277-amino acid protein has a calculated molecular mass of 31 kDa. UCK1 belongs to the uridine kinase family and UCK have important roles for the phosphorylation of nucleoside analogs that may be important in chemotherapy of cancer.

Publikationen

Species	Application	Title
human	WB	Int J Cancer; Adult T-cell leukemia-lymphoma acquires resistance to DNA demethylating agents through dysregulation of enzymes involved in pyrimidine metabolism.; Authors - Nao Yoshida-Sakai
human,mouse	WB	Blood Adv; Reprogramming of pyrimidine nucleotide metabolism supports vigorous cell proliferation of normal and malignant T cells; Authors - Tatsuro Watanabe

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

H (Verified Customer) (07-09-2020)

This antibody is good considering the low expression of UCK1 protein. I could see a thick band that corresponds with UCK1 MW.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

UCK1 Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:12271-1-AP)