UCHL3 Polyklonaler Antikörper

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• KD/KO Validated

UCHL3 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, IHC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Kat-Nr. : 12384-1-AP

Synonyme

Ubiquitin carboxyl terminal hydrolase isozyme L3, Ubiquitin thioesterase L3, UCHL3, UCH-L3

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Galerie der Validierungsdaten

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  WB analysis using 12384-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12384-1-AP (UCHL3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat spleen using 12384-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 12384-1-AP (UCHL3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 12384-1-AP

  IP result of anti-UCHL3 (IP:12384-1-AP, 4ug; Detection:12384-1-AP 1:3000) with HeLa cells lysate 1320 ug.

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  IHC staining of human pancreas cancer using 12384-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12384-1-AP (UCHL3 antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen, Rattenmilzgewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 8 publications below
• WB: See 11 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below

Produktinformation

12384-1-AP bindet in WB, IP, IF, IHC, CoIP, ELISA UCHL3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UCHL3 fusion protein Ag3053
• Vollständiger Name: ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)
• Berechnetes Molekulargewicht: 26 kDa
• Beobachtetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: BC018125
• Gene symbol: UCHL3
• Gene ID (NCBI): 7347
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UCH-L3 belongs to the ubiquitin C-terminal hydrolase family that deubiquitinates ubiquitin-protein conjugates in the ubiquitin-proteasome system. This enzyme indirectly increases the phosphorylation of IGFIR, AKT, FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. It is required for stress-response retinal, skeletal muscle and germ cell maintenance and may be involved in working memory.

Publikationen

Species	Application	Title
human,mouse	WB	Nat Commun; Farrerol directly activates the deubiqutinase UCHL3 to promote DNA repair and reprogramming when mediated by somatic cell nuclear transfer; Authors - Weina Zhang ; KD Validated
human	WB	Mol Cell; An Interaction Landscape of Ubiquitin Signaling.; Authors - Xiaofei Zhang
human	WB	Nat Commun; Structural basis for specific inhibition of the deubiquitinase UCHL1; Authors - Christian Grethe ; KD Validated
human	WB	Genes Dev; A phosphorylation-deubiquitination cascade regulates the BRCA2-RAD51 axis in homologous recombination.; Authors - Kuntian Luo ; KO Validated
human	WB,IHC	Am J Cancer Res; UCHL3 promotes pancreatic cancer progression and chemo-resistance through FOXM1 stabilization.; Authors - Zhiwang Song ; KD Validated
mouse	WB	Sci Rep; Ubiquitin C-terminal hydrolase L1 (UCHL1) regulates post-myocardial infarction cardiac fibrosis through glucose-regulated protein of 78 kDa (GRP78).; Authors - Qian Lei