UBE2S Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

UBE2S Polyklonal Antikörper für FC, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 14115-1-AP

Synonyme

E2 EPF, E2EPF, EPF5, UBE2S, Ubiquitin carrier protein S, Ubiquitin protein ligase S

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Galerie der Validierungsdaten

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  WB analysis using 14115-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14115-1-AP (UBE2S antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14115-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14115-1-AP (UBE2S antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 14115-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 14115-1-AP (UBE2S antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MDA-MB-453s using 14115-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 14115-1-AP (UBE2S antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 14115-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 14115-1-AP (UBE2S antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

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  WB analysis of HeLa using 14115-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14115-1-AP (UBE2S antibody) at dilution of 1:1500 incubated at 4 degree celsius over night.

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  WB analysis of HEK-293T using 14115-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 14115-1-AP (UBE2S antibody) at dilution of 1:1500 incubated at 4 degree celsius over night.

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  WB analysis of mouse liver using 14115-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14115-1-AP (UBE2S antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A2780 using 14115-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 14115-1-AP (UBE2S antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of A2780 using 14115-1-AP

  IP Result of anti-UBE2S (IP:14115-1-AP, 4ug; Detection:14115-1-AP 1:1000) with A2780 cells lysate 960ug.

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  IHC staining of human liver cancer using 14115-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14115-1-AP (UBE2S antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 14115-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14115-1-AP (UBE2S antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 14115-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 14115-1-AP (UBE2S antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary tumor using 14115-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 14115-1-AP (UBE2S antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 14115-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14115-1-AP (UBE2S antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 14115-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14115-1-AP (UBE2S antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 14115-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14115-1-AP (UBE2S antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 14115-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14115-1-AP (UBE2S antibody) at dilution of 1:50 (under 40x lens).

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  FC experiment of Jurkat using 14115-1-AP

  1X10^6 Jurkat cells were stained with 0.5ug UBE2S antibody (14115-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, A2780-Zellen, HEK-293T-Zellen, HEK-293-Zellen, HeLa-Zellen, humanes Lebergewebe, Mauslebergewebe, MDA-MB-453s-Zellen, MOLT-4-Zellen, PC-3-Zellen
• Erfolgreiche IP: A2780-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Hautgewebe, humanes Mammakarzinomgewebe, humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 6 publications below
• WB: See 8 publications below
• IHC: See 5 publications below
• IF: See 1 publications below
• CoIP: See 3 publications below

Produktinformation

14115-1-AP bindet in WB, IP, IHC, IF, FC, CoIP, ELISA UBE2S und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UBE2S fusion protein Ag5264
• Vollständiger Name: ubiquitin-conjugating enzyme E2S
• Berechnetes Molekulargewicht: 222 aa, 24 kDa
• Beobachtetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: BC066948
• Gene symbol: UBE2S
• Gene ID (NCBI): 27338
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UBE2S(Ubiquitin-conjugating enzyme E2 S) is also named as E2EPF and belongs to the ubiquitin-conjugating enzyme family. It acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C) and a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Overexpression of UBE2S can increase tumor cell proliferation, invasion, and metastasis through the VHL-HIF pathway (PMID:16819549).

Publikationen

Species	Application	Title
mouse	WB	Protein Cell; BMP4 preserves the developmental potential of mESCs through Ube2s- and Chmp4b-mediated chromosomal stability safeguarding.; Authors - Mingzhu Wang ; KD Validated
human	WB,IHC	Int J Biol Sci; UBE2S as a novel ubiquitinated regulator of p16 and β-catenin to promote bone metastasis of prostate cancer.; Authors - Shengmeng Peng ; KD Validated
human	WB	Int J Biol Sci; The Role of CHK1 Varies with the Status of Oestrogen-receptor and Progesterone-receptor in the Targeted Therapy for Breast Cancer.; Authors - Wei Xu
human	WB,IHC	J Ovarian Res; UBE2S promotes the progression and Olaparib resistance of ovarian cancer through Wnt/β-catenin signaling pathway.; Authors - Wenjing Hu
human	WB,IHC	Biochem Biophys Res Commun; UBE2S associated with OSCC proliferation by promotion of P21 degradation via the ubiquitin-proteasome system.; Authors - Shusaku Yoshimura ; KD Validated
human	WB,CoIP	Cell Rep; Ubiquitin-Conjugating Enzyme 2S Enhances Viral Replication by Inhibiting Type I IFN Production through Recruiting USP15 to Deubiquitinate TBK1.; Authors - Li Huang ; KO Validated