UBE2B Polyklonaler Antikörper

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• KD/KO Validated

UBE2B Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 10733-1-AP

Synonyme

E2 17kDa, HHR6B, HR6B, RAD6 homolog B, RAD6B, UBC2, UBE2B, Ubiquitin carrier protein B, Ubiquitin protein ligase B

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Galerie der Validierungsdaten

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  WB analysis of L02 using 10733-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 10733-1-AP (UBE2B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Transfected HEK-293 using 10733-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 10733-1-AP (UBE2B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 10733-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10733-1-AP (UBE2B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 10733-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10733-1-AP (UBE2B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: L02-Zellen, Transfizierte HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

10733-1-AP bindet in WB, IF, IHC, ELISA UBE2B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UBE2B fusion protein Ag1147
• Vollständiger Name: ubiquitin-conjugating enzyme E2B (RAD6 homolog)
• Berechnetes Molekulargewicht: 17 kDa
• Beobachtetes Molekulargewicht: 17 kDa
• GenBank-Zugangsnummer: BC005979
• Gene symbol: UBE2B
• Gene ID (NCBI): 7320
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UBE2B (Ubiquitin-conjugating enzyme E2 B) is also named as RAD6B and belongs to the ubiquitin-conjugating enzyme family. Rad6B is an E2-conjugating enzyme, reported to mediate beta-catenin polyubiquitination and stabilization, thus activating the Wnt signaling pathway (PMID:21118991). The gene encodes a 152 amino acid protein. The Rad6B and Cdc34 proteins are significantly elevated in meiotic and postmeiotic haploid germ cells when chromatin modifications occur (PMID:10373550).

Publikationen

Species	Application	Title
mouse	WB,IF	Biol Reprod; Identification of Ube2b as a Novel Target of Androgen Receptor in Mouse Sertoli Cells.; Authors - Mou Lisha L ; KD Validated
		Int J Mol Med; Identification of NR0B1 as a novel androgen receptor co-repressor in mouse Sertoli cells.; Authors - Yu-Chi Li
human	WB,IHC,IF	Bioengineered; UBE2B promotes ovarian cancer growth via promoting RAD18 mediated ZMYM2 monoubiquitination and stabilization.; Authors - Xi Zeng ; KD Validated
human	WB	Nat Commun; Auranofin targets UBA1 and enhances UBA1 activity by facilitating ubiquitin trans-thioesterification to E2 ubiquitin-conjugating enzymes; Authors - Wenjing Yan