UBE2A Polyklonaler Antikörper

UBE2A Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Rezensionen (1)

Kat-Nr. : 11080-1-AP

Synonyme

HHR6A, HR6A, RAD6 homolog A, RAD6A, UBC2, UBE2A, Ubiquitin carrier protein A, Ubiquitin protein ligase A

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 11080-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11080-1-AP (UBE2A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 11080-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 11080-1-AP (UBE2A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 11080-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 11080-1-AP (UBE2A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 11080-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 11080-1-AP (UBE2A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human nasopharyngeal carcinoma using 11080-1-AP

  Immunohistochemical analysis of paraffin-embedded human nasopharyngeal carcinoma tissue slide using 11080-1-AP (UBE2A antibody) at dilution of 1:200 (under 40x lens)..

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, COLO 320-Zellen, humanes Nierengewebe, humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: humanes Nasopharynxkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

11080-1-AP bindet in WB, IHC, ELISA UBE2A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UBE2A fusion protein Ag1567
• Vollständiger Name: ubiquitin-conjugating enzyme E2A (RAD6 homolog)
• Berechnetes Molekulargewicht: 17 kDa
• Beobachtetes Molekulargewicht: 17 kDa
• GenBank-Zugangsnummer: BC010175
• Gene symbol: UBE2A
• Gene ID (NCBI): 7319
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UBE2A(Ubiquitin-conjugating enzyme E2 A) serves as the cognate E2-conjugating enzyme. It plays a critical role in regulating TP53 protein levels under both normal and stress conditions. UBE2A, UBE2B regulates TP53 levels in a "yin-yang" manner through a combination of two distinct mechanisms in mammalian cells(PMID:22083959). This protein has 3 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
human	WB	J Cell Sci; A specific subset of E2 ubiquitin-conjugating enzymes regulate Parkin activation and mitophagy differently.; Authors - Fabienne C Fiesel
rat	WB	Mol Med Rep; Yuan‑zhi‑san inhibits tau protein aggregation in an Aβ1‑40‑induced Alzheimer's disease rat model via the ubiquitin‑proteasome system.; Authors - Bin Li
mouse	WB	Cell Rep; A program of successive gene expression in mouse one-cell embryos; Authors - Maki Asami

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Alexandra (Verified Customer) (03-20-2024)

I was searching for a Rad6 antibody is strong enough to visualize endogenous protein. This is it. Works pretty good with endogenous Rad6 and extremely good with transfected cell lisate (for that I recomend a higher dilution, with 1000x the signal can be too strong)

• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Cell culture
• Primary Antibody Dilution: 1000x
• Cell Tissue Type: U2OS human osteosarcoma cell line

UBE2A Antibody Western Blot,Immunohistochemistry,Immunofluorescence,Immunoprecipitation,Cell culture validation (1000x dilution) in U2OS human osteosarcoma cell line (Cat no:11080-1-AP)