UBAC1 Polyklonaler Antikörper

UBAC1 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19844-1-AP

Synonyme

GBDR1, KPC2, RP11 432J22.3, UBA domain containing 1, UBAC1, UBADC1

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Galerie der Validierungsdaten

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  WB analysis using 19844-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19844-1-AP (UBAC1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 19844-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19844-1-AP (UBAC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 19844-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 19844-1-AP (UBAC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 19844-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 19844-1-AP (UBAC1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 19844-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19844-1-AP (UBAC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 19844-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 19844-1-AP (UBAC1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human heart using 19844-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19844-1-AP (UBAC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Mausherzgewebe, Rattenherzgewebe, Rattenhirngewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

19844-1-AP bindet in WB, IHC,ELISA UBAC1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UBAC1 fusion protein Ag13887
• Vollständiger Name: UBA domain containing 1
• Berechnetes Molekulargewicht: 405 aa, 45 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: BC004967
• Gene symbol: UBAC1
• Gene ID (NCBI): 10422
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UBAC1(Ubiquitin-associated domain-containing protein 1) is also named as GBDR1, KPC2, UBADC1. It has several putative phosphorylation sites, including 8 CK2 (see 115440) sites, as well as 1 N-glycosylation site and 2 N-myristoylation sites(PMID:10857748). It is required for poly-ubiquitination and proteasome-mediated degradation of CDKN1B during G1 phase of the cell cycle.Northern blot analysis shows UBAC1 expression with the highest levels in heart and skeletal muscle and lowest level in lung. This antibody is specific to UBAC1.