TWF1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TWF1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 11732-1-AP

Synonyme

Twinfilin-1, Twinfilin 1

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Galerie der Validierungsdaten

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  WB analysis using 11732-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11732-1-AP (TWF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 11732-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11732-1-AP (TWF1 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11732-1-AP

  WB result of TWF1 antibody (11732-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TWF1 transfected HeLa cells.

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  IHC staining of human lung cancer using 11732-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11732-1-AP (TWF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 11732-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11732-1-AP (TWF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 11732-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TWF1 antibody (11732-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: C6-Zellen, C2C12-Zellen, HeLa-Zellen, Maushodengewebe, NIH/3T3-Zellen, Rattenhodengewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:14000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

11732-1-AP bindet in WB, IHC, IF/ICC, CoIP, ELISA TWF1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TWF1 fusion protein Ag2332
• Vollständiger Name: twinfilin, actin-binding protein, homolog 1 (Drosophila)
• Berechnetes Molekulargewicht: 368 aa, 42 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC022344
• Gene symbol: TWF1
• Gene ID (NCBI): 5756
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,IF	Biochem Biophys Res Commun; Twinfilin 1 enhances milk bio-synthesis and proliferation of bovine mammary epithelial cells via the mTOR signaling pathway.; Authors - Lu Li ; KD Validated
human	WB	J Thorac Dis; Overexpression of TWF1 promotes lung adenocarcinoma progression and is associated with poor prognosis in cancer patients through the MMP1 signaling pathway; Authors - Kui Zhai ; KD Validated
human,mouse	WB,IHC,CoIP	FASEB J; TWF1 induces autophagy and accelerates malignant phenotype in lung adenocarcinoma via inhibiting the cAMP signaling pathway; Authors - Yu Wang ; KD Validated
mouse	WB	Sci Signal; The actin-binding protein CAP1 represses MRTF-SRF-dependent gene expression in mouse cerebral cortex; Authors - Sharof Khudayberdiev