TUSC2 Polyklonaler Antikörper

TUSC2 Polyklonal Antikörper für WB, IF, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 11538-1-AP

Synonyme

C3orf11, Fus 1 protein, FUS1, Fusion 1 protein, LGCC, PAP, PDAP2, PDGFA associated protein 2, tumor suppressor candidate 2, TUSC2

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Galerie der Validierungsdaten

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  WB analysis of human heart using 11538-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11538-1-AP (TUSC2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 11538-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 11538-1-AP (TUSC2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 11538-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11538-1-AP (TUSC2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung cancer using 11538-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11538-1-AP (TUSC2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon cancer using 11538-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11538-1-AP (TUSC2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 11538-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11538-1-AP (TUSC2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 11538-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11538-1-AP (TUSC2 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of A549 using 11538-1-AP

  Immunofluorescent analysis of A549 cells using 11538-1-AP (TUSC2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Herzgewebe, Maus-Pankreasgewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:300-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

11538-1-AP bindet in WB, IF, IHC, ELISA TUSC2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TUSC2 fusion protein Ag2118
• Vollständiger Name: tumor suppressor candidate 2
• Berechnetes Molekulargewicht: 12 kDa
• Beobachtetes Molekulargewicht: 10-12 kDa
• GenBank-Zugangsnummer: BC023976
• Gene symbol: TUSC2
• Gene ID (NCBI): 11334
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FUS1 (or TUSC2) gene is a highly conserved lung cancer candidate gene, which was identified in the 120 kb 3p21.3 critical region contained in nested lung and breast cancer homozygous deletions. Overexpression of FUS1 gene leads to G1 arrest and growth inhibition of lung cancer cells (PMID: 11593436). The encoded Fusion-1 protein was down-regulated, mutated or lost in the majority of inflammatory thoracic malignancies. It has been evidenced that Fusion-1 establishes its immune- and tumour-suppressive activities via regulation of mitochondrial homeostasis (PMID: 22513871). In addition, myristoylation is found to be required for Fusion-1-mediated tumor-suppressing activity and suggest a novel mechanism for the inactivation of tumor suppressors in lung cancer and a role for deficient posttranslational modification in tumor suppressor-gene-mediated carcinogenesis (PMID: 15126327).

Publikationen

Species	Application	Title
human,mouse	WB,IHC,IF	Cancer Lett; NEDD4 degrades TUSC2 to promote glioblastoma progression.; Authors - Tadas K Rimkus
human	WB,IHC	Oncotarget; TWIST1/miR-584/TUSC2 pathway induces resistance to apoptosis in thyroid cancer cells.; Authors - Francesca Maria Orlandella