TUBB3-specific/TUJ1 Monoklonaler Antikörper

TUBB3-specific/TUJ1 Monoklonal Antikörper für WB, IF-P

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF-P

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1F8G10

Kat-Nr. : CL488-66375

Synonyme

TUBB3, TUBB3-specific, TUJ1, beta tubulin, Tubulin beta 3 chain

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  WB analysis using CL488-66375

  Neuro-2a cell lysates were subjected to SDS PAGE followed by western blot with CL488-66375 (TUBB3-specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse brain using CL488-66375

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CL488-66375 (TUBB3-specific antibody) at dilution of 1:50.

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  IF Staining of mouse brain using CL488-66375

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CL488-66375 (TUBB3-specific antibody) at dilution of 1:50.

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  IF Staining of mouse spine using CL488-66375

  E14.5 FFPE mouse spine stained for beta-III tubulin (red, Cat. No CL488-66375) and GFAP (cyan, Cat. No 60190-1-Ig). Beta-III tubulin stains neurons and was conjugated to CoraLite-488 fluorescent dye and pseudocolored to red. GFAP stains astrocytes along the spinal column. In this image, astrocyte projections can be seen among spinal neurons in the spinal cord. Image credit: @Immunofluorescence on Instagram.

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  IF Staining of mouse cerebellum using CL488-66375

  Neonatal mouse cerebellum stained for beta-III tubulin (magenta, Cat. No CL488-66375) and GFAP (green, Cat. No 60190-1-Ig). Beta-III tubulin stains neurons and was conjugated to CoraLite-488 fluorescent dye and pseudocolored to magenta. GFAP stains astrocytes. In this image, astrocyte projections can be seen intermingling with neurons. Image credit: @Immunofluorescence on Instagram.

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  IF Staining of mouse testis using CL488-66375

  Adult mouse testes were co-stained with TUBB3 (CL488-66375, in green), DDX4/VASA (51042-1-AP, in magenta), and PCNA (60097-1-Ig, in blue). TUBB3/Beta III tubulin marks the Sertoli cells (structural support cells), VASA marks the germ cells (developing sperm), and PCNA marks proliferating cells (these cells are at the base of the tubule, where the sperm stem cells are located). The image was created in paid partnership with @Immunofluorescence on Instagram.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Neuro-2a-Zellen
• Erfolgreiche Detektion in IF-P: Maushirngewebe, Maus-Cerebellum-Gewebe, Maushodengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-66375 bindet in WB, IF-P TUBB3-specific/TUJ1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: tubulin, beta 3
• Berechnetes Molekulargewicht: 55 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: NM_001197181
• Gene symbol: TUBB3
• Gene ID (NCBI): 10381
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TUBB3, the class III β tubulin or Tuj1, is selectively expressed in testis and neurons of the central and peripheral nervous system. It has been widely used as a marker for neurons. Aberrant expression of TUBB3 has also been found in various tumors of non-neural origin and can be used as a biomarker for cancer aggressiveness and a marker for the tendency to respond poorly to chemotherapy. This antibody is specific to TUBB3 but not cross-react with other tubulin isoforms. And the antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.