Prealbumin/transthyretin Monoklonaler Antikörper

Prealbumin/transthyretin Monoklonal Antikörper für FC, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

CloneNo.

1H8C4

Kat-Nr. : 66108-1-Ig

Synonyme

ATTR, HsT2651, PALB, Prealbumin, Prealbumin/transthyretin, TBPA, transthyretin, TTR

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Galerie der Validierungsdaten

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  WB analysis of human brain using 66108-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 66108-1-Ig (Prealbumin/transthyretin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 66108-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 66108-1-Ig (Prealbumin/transthyretin antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 66108-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66108-1-Ig (Prealbumin/transthyretin antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 66108-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66108-1-Ig (Prealbumin/transthyretin antibody at dilution of 1:50.

• 

  IHC staining of mouse liver using 66108-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66108-1-Ig (Prealbumin/transthyretin antibody at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 66108-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66108-1-Ig (Prealbumin/transthyretin antibody at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 66108-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66108-1-Ig (Prealbumin/transthyretin antibody at dilution of 1:50.

• 

  FC experiment of PC-12 using 66108-1-Ig

  1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human Prealbumin/transthyretin (66108-1-Ig, Clone:1H8C4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Human brain, humanes Herzgewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: PC-12-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

66108-1-Ig bindet in WB, IHC, FC, ELISA Prealbumin/transthyretin und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Prealbumin/transthyretin fusion protein Ag19232
• Vollständiger Name: transthyretin
• Berechnetes Molekulargewicht: 147 aa, 16 kDa
• Beobachtetes Molekulargewicht: 14-16 kDa, 28-33 kDa
• GenBank-Zugangsnummer: BC020791
• Gene symbol: TTR
• Gene ID (NCBI): 7276
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Transthyretin (TTR) is a plasma transport protein for thyroxine and retinol, through the association with retinol-binding protein. It is a homotetrameric protein synthesized mainly in liver, choroid plexus, retinal pigment epithelium, and pancreas.Within the CNS, TTR is the only known protein synthesized solely by the choroid plexus. Mutant and wildtype TTR give rise to various forms of amyloid deposition (amyloidosis). Defects in TTR are the cause of amyloidosis transthyretin-related (AMYL-TTR),hyperthyroxinemia dystransthyretinemic euthyroidal (HTDE) and carpal tunnel syndrome type 1 (CTS1). In addition, positive immunostaining for TTR has been reported as a sensitive diagnostic marker of choroid plexus tumors. TTR can be expressed as monomer (14-16 kDa), dimmer (28-33 kDa), or tetramer around 55-60 kDa. (PMID: 22103483)

Publikationen

Species	Application	Title
human	WB	Nat Commun; Secondary structure RNA elements control the cleavage activity of DICER.; Authors - Trung Duc Nguyen
mouse	WB	Am J Pathol; Renal function in aged C57BL/6J mice is impaired by deposition of age-related apolipoprotein A-II amyloid independent of kidney aging; Authors - Ying Li