Prealbumin/transthyretin Polyklonaler Antikörper

Prealbumin/transthyretin Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen

25

Rezensionen (1)

Kat-Nr. : 11891-1-AP

Synonyme

Prealbumin, transthyretin, TTR, TBPA, PALB

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Galerie der Validierungsdaten

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  WB analysis using 11891-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11891-1-AP (Prealbumin/transthyretin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human spleen using 11891-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 11891-1-AP (Prealbumin/transthyretin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 11891-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11891-1-AP (Prealbumin/transthyretin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11891-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11891-1-AP (Prealbumin/transthyretin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 11891-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11891-1-AP (Prealbumin/transthyretin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11891-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11891-1-AP (Prealbumin/transthyretin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11891-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11891-1-AP (Prealbumin/transthyretin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11891-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11891-1-AP (Prealbumin/transthyretin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11891-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11891-1-AP (Prealbumin/transthyretin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11891-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11891-1-AP (Prealbumin/transthyretin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human plasma using 11891-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 11891-1-AP (Prealbumin/transthyretin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human plasma using 11891-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 11891-1-AP (Prealbumin/transthyretin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human plasma using 11891-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 11891-1-AP (Prealbumin/transthyretin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 11891-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11891-1-AP (Prealbumin/transthyretin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 11891-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 11891-1-AP (Prealbumin/transthyretin antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver cancer using 11891-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 11891-1-AP (Prealbumin/transthyretin antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 11891-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11891-1-AP (Prealbumin/transthyretin antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 11891-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11891-1-AP (Prealbumin/transthyretin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 11891-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11891-1-AP (Prealbumin/transthyretin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 11891-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Prealbumin/transthyretin antibody (11891-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: human plasma, HEK-293-Zellen, humanes Herzgewebe, humanes Hirngewebe, humanes Lebergewebe, humanes Milzgewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Nierengewebe, Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 13 publications below
• IHC: See 7 publications below
• IF: See 4 publications below

Produktinformation

11891-1-AP bindet in WB, IHC, IF-P, ELISA Prealbumin/transthyretin und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Prealbumin/transthyretin fusion protein Ag2499
• Vollständiger Name: transthyretin
• Berechnetes Molekulargewicht: 147 aa, 16 kDa
• Beobachtetes Molekulargewicht: 16 kDa
• GenBank-Zugangsnummer: BC020791
• Gene symbol: TTR
• Gene ID (NCBI): 7276
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Transthyretin (TTR) is a plasma transport protein for thyroxine and retinol, through the association with retinol-binding protein. It is a homotetrameric protein synthesized mainly in liver, choroid plexus, retinal pigment epithelium, and pancreas.Within the CNS, TTR is the only known protein synthesized solely by the choroid plexus. Mutant and wildtype TTR give rise to various forms of amyloid deposition (amyloidosis). Defects in TTR are the cause of amyloidosis transthyretin-related (AMYL-TTR),hyperthyroxinemia dystransthyretinemic euthyroidal (HTDE) and carpal tunnel syndrome type 1 (CTS1). In addition, positive immunostaining for TTR has been reported as a sensitive diagnostic marker of choroid plexus tumors. However Western blot analysis using specific rabbit anti-mouse TTR reveals that TTR is absent from the cortex, hippocampus, and cerebellum and present in the choroid plexus. (PMID: 22103483, 16698124)

Publikationen

Species	Application	Title
	IF	Cell; Ex utero monkey embryogenesis from blastocyst to early organogenesis; Authors - Yandong Gong
mouse	IHC	Dev Cell; Spatially dependent dynamic MAPK modulation by the Nde1-Lis1-Brap complex patterns mammalian CNS.; Authors - Alison A Lanctot
human	IF	Dev Cell; Lysosomal dynamics regulate mammalian cortical neurogenesis; Authors - Wenzheng Zou
mouse	WB	Theranostics; Growth hormone receptor disrupts glucose homeostasis via promoting and stabilizing retinol binding protein 4.; Authors - Jinxin Liu
	WB	Diabetes; PMID: 25524914; Authors - Laura Zemany
human	WB	Int J Mol Sci; Plasma Exosome Profile in ST-Elevation Myocardial Infarction Patients with and without Out-of-Hospital Cardiac Arrest.; Authors - Marta Zarà

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kenzo (Verified Customer) (11-11-2023)

This antibody worked for mouse heart tissue sections with little background.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Heart