TTK Polyklonaler Antikörper

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• KD/KO Validated

TTK Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Rezensionen (1)

Kat-Nr. : 10381-1-AP

Synonyme

MPS1L1, MPS1, Mps 1, hMPS1, ESK

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Galerie der Validierungsdaten

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  WB analysis using 10381-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10381-1-AP (TTK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10381-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10381-1-AP (TTK Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10381-1-AP

  WB result of TTK antibody (10381-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-TTK transfected HEK-293 cells.

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  IP experiment of DU 145 using 10381-1-AP

  IP result of anti-TTK (IP:10381-1-AP, 4ug; Detection:10381-1-AP 1:600) with DU 145 cells lysate 1280 ug.

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  IHC staining of human breast cancer using 10381-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10381-1-AP (TTK Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 10381-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 10381-1-AP (TTK antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 10381-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10381-1-AP (TTK antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 10381-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10381-1-AP (TTK antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human kidney using 10381-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10381-1-AP (TTK antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 10381-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10381-1-AP (TTK antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver cancer using 10381-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10381-1-AP (TTK antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10381-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10381-1-AP (TTK antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 10381-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TTK antibody (10381-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 10381-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human TTK (10381-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: DU 145-Zellen, HEK-293-Zellen, HeLa-Zellen, MCF-7-Zellen
• Erfolgreiche IP: DU 145-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Hodengewebe, humanes Leberkarzinomgewebe, humanes Nierengewebe, Maus-Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 5 publications below
• IF: See 3 publications below

Produktinformation

10381-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA TTK und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TTK fusion protein Ag0405
• Vollständiger Name: TTK protein kinase
• Berechnetes Molekulargewicht: 97 kDa
• Beobachtetes Molekulargewicht: 96-97 kDa
• GenBank-Zugangsnummer: BC000633
• Gene symbol: TTK
• Gene ID (NCBI): 7272
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mps1 family of kinases colocalizes with mitotic checkpoint proteins in kinetochores and/or spindle pole bodies/centrosomes. TTK has been shown to be a cell cycle-regulated kinase displaying maximum activity during M phase(PMID:15618221). Its kinase activity is required for proper chromosome segregation during mitosis through its involvements in microtubule-chromosome attachment error correction and the mitotic checkpoint(PMID:20937696). TTK mRNA is present at relatively high levels in testis and thymus, tissues which contain a large number of proliferating cells, but is not detected in most other benign tissues (PMID:1639825). It has 2 isoforms produced by alternative splicing (PMID:25137017).

Publikationen

Species	Application	Title
human	IF	J Cell Biol; PP6 regulation of Aurora A-TPX2 limits NDC80 phosphorylation and mitotic spindle size; Authors - Tomoaki Sobajima
		Oxid Med Cell Longev; Oxidative Stress Delays Prometaphase/Metaphase of the First Cleavage in Mouse Zygotes via the MAD2L1-Mediated Spindle Assembly Checkpoint.; Authors - Que Wu
human	WB,IF	J Nanobiotechnology; GRP75-driven, cell-cycle-dependent macropinocytosis of Tat/pDNA-Ca2+ nanoparticles underlies distinct gene therapy effect in ovarian cancer.; Authors - Linjia Su
human	IHC	Int J Cancer; Development and validation of a five-gene model to predict postoperative brain metastasis in operable lung adenocarcinoma.; Authors - Fangqiu Fu
human	IHC	Front Oncol; Identification and Validation of Hub Genes Associated with Bladder Cancer by Integrated Bioinformatics and Experimental Assays; Authors - Kang Chen
human	IHC	Front Mol Biosci; Characteristic Analysis of Featured Genes Associated With Stemness Indices in Colorectal Cancer.; Authors - Yongqu Lu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Zhiping (Verified Customer) (07-28-2022)

Very clear band

• Applications: Western Blot
• Primary Antibody Dilution: 1:500