TSPAN13 Polyklonaler Antikörper

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• KD/KO Validated

TSPAN13 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Rezensionen (1)

Kat-Nr. : 13570-1-AP

Synonyme

NET 6, NET6, Tetraspan NET 6, tetraspanin 13, TM4SF13, Tspan 13, TSPAN13

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Galerie der Validierungsdaten

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  WB analysis of mouse lung using 13570-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13570-1-AP (TSPAN13 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 13570-1-AP

  IP result of anti-TSPAN13 (IP:13570-1-AP, 3ug; Detection:13570-1-AP 1:300) with mouse lung tissue lysate 4000ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslungengewebe
• Erfolgreiche IP: Mauslungengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below

Produktinformation

13570-1-AP bindet in WB, IP, ELISA TSPAN13 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TSPAN13 fusion protein Ag4464
• Vollständiger Name: tetraspanin 13
• Berechnetes Molekulargewicht: 204 aa, 22 kDa
• Beobachtetes Molekulargewicht: 28-35 kDa
• GenBank-Zugangsnummer: BC033863
• Gene symbol: TSPAN13
• Gene ID (NCBI): 27075
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TSPAN13 (also known as NET6 or TM4SF13) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Many of these members have been implicated in signal transduction, cell-cell interactions, and cellular activation and development. Overexpression of TSPAN13 has been reported in prostate cancer, suggesting that TSPAN13 may have an important role in the progression of prostate cancer (PMID: 19148481). TSPAN13 has a calcular molecular weight of 22 kDa. This polyclonal antibody is raised against 100-204aa of human TSPAN13. The experimentally determined molecular mass of 28-35 kDa is larger than the calculated molecular mass, which could be a result of post-translational modifications (PMID: 23648579).

Publikationen

Species	Application	Title
human	WB	Bosn J Basic Med Sci; Downregulation of TSPAN13 by miR-369-3p inhibits cell proliferation in papillary thyroid cancer (PTC).; Authors - Peng Li ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jerome (Verified Customer) (07-08-2020)

Sign background, unclear if specific.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

TSPAN13 Antibody Western Blot, validation (1:1000 dilution) in HEK293T (Cat no:13570-1-AP)