TSEN2 Polyklonaler Antikörper

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• KD/KO Validated

TSEN2 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 13103-2-AP

Synonyme

tRNA-intron endonuclease Sen2, SEN2L, SEN2, PCH2B, HsSen2

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Galerie der Validierungsdaten

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  WB analysis of mouse colon using 13103-2-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 13103-2-AP (TSEN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 13103-2-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 13103-2-AP (TSEN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 13103-2-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13103-2-AP (TSEN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 13103-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13103-2-AP (TSEN2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 13103-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 13103-2-AP (TSEN2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Kolongewebe, humanes Hirngewebe, Maushirngewebe
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below

Produktinformation

13103-2-AP bindet in WB, IF/ICC, ELISA TSEN2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TSEN2 fusion protein Ag3742
• Vollständiger Name: tRNA splicing endonuclease 2 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 465 aa, 51 kDa
• Beobachtetes Molekulargewicht: 51 kDa
• GenBank-Zugangsnummer: BC019582
• Gene symbol: TSEN2
• Gene ID (NCBI): 80746
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TSEN2, also named as SEN2, belongs to the tRNA-intron endonuclease family. TSEN2 is a metal ion independent nuclease that cleave the 5' splice site (PMID: 32476018). TSEN2 has 5 isoforms with the molecular mass of 45-53 kDa. TSEN2 is involved in the formation of a human endonuclease complex whose function is the removal of introns from pre-tRNA (PMID: 16595010).

Publikationen

Species	Application	Title
human	WB	Cell; CLP1 Founder Mutation Links tRNA Splicing and Maturation to Cerebellar Development and Neurodegeneration.; Authors - Ashleigh E Schaffer
human	WB	Am J Hum Genet; Autosomal-Recessive Mutations in the tRNA Splicing Endonuclease Subunit TSEN15 Cause Pontocerebellar Hypoplasia and Progressive Microcephaly.; Authors - Martin W Breuss
human	WB	Genetics; An unknown essential function of tRNA splicing endonuclease is linked to the integrated stress response and intron debranching; Authors - Jennifer E Hurtig ; KD Validated