TSC22D1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TSC22D1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 10214-1-Ig

Synonyme

RP11 269C23.2, Regulatory protein TSC-22, Regulatory protein TSC 22, KIAA1994, HUCEP-2

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Galerie der Validierungsdaten

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  WB analysis of human brain using 10214-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 10214-1-Ig (TSC22D1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 10214-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 10214-1-Ig (TSC22D1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 10214-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10214-1-Ig (TSC22D1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of rat brain using 10214-1-Ig

  IP result of anti-TSC22D1 (IP:10214-1-Ig, 4ug; Detection:10214-1-Ig 1:500) with rat brain tissue lysate 4000ug.

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  IHC staining of human testis using 10214-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10214-1-Ig (TSC22D1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 10214-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10214-1-Ig (TSC22D1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human gliomas using 10214-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10214-1-Ig (TSC22D1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human gliomas using 10214-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10214-1-Ig (TSC22D1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 10214-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10214-1-Ig (TSC22D1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 10214-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10214-1-Ig (TSC22D1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of A549 using 10214-1-Ig

  Immunofluorescent analysis of (4% PFA ) fixed A549 cells using 10214-1-Ig(TSC22D1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, A549-Zellen, Rattenhirngewebe
• Erfolgreiche IP: Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 9 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• IP: See 2 publications below

Produktinformation

10214-1-Ig bindet in WB, IHC, IF/ICC, IP, ELISA TSC22D1 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TSC22D1 fusion protein Ag0287
• Vollständiger Name: TSC22 domain family, member 1
• Berechnetes Molekulargewicht: 16 kDa
• Beobachtetes Molekulargewicht: 16 kDa
• GenBank-Zugangsnummer: BC000456
• Gene symbol: TSC22D1
• Gene ID (NCBI): 8848
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TSC22 domain family 1, transforming growth factor beta 1 induced transcript 4, (TSC22D1, synonyms: TSC22, TGFB1I4, MGC17597) is a member of a family of leucine zipper containing transcription factors with repressor activity and belongs to the large family of early response genes. TSC-22 regulates cell growth and differentiation, and cell death. Leucine zipper structure of TSC22 inhibits the anchorage independent growth of cancer cells. TSC-22 is also an important downstream component of peroxisome proliferator-activated receptor (PPAR) and TGF-beta signaling during intestinal epithelial cell differentiation. This antibody is a rabbit polyclonal antibody raised against an internal region of human TSC22D1.

Publikationen

Species	Application	Title
mouse	WB	Cell Death Differ; TSC-22D1 isoforms have opposing roles in mammary epithelial cell survival.; Authors - Huser C A CA
human,mouse	WB,IHC	Clin Cancer Res; Transforming Growth Factor-{beta}-Stimulated Clone-22 Is an Androgen-Regulated Gene That Enhances Apoptosis in Prostate Cancer following Insulin-Like Growth Factor-I Receptor Inhibition.; Authors - Sprenger Cynthia C T CC ; KD Validated
human	WB,IF	Int J Mol Sci; Identification of Binding Proteins for TSC22D1 Family Proteins Using Mass Spectrometry.; Authors - Ryouta Kamimura
mouse	IP,WB	FEBS J; Multiple TSC22D4 iso-/phospho-glycoforms display idiosyncratic subcellular localizations and interacting protein partners.; Authors - Canterini Sonia S
human	WB,IF	Am J Physiol Heart Circ Physiol; Transforming growth factor-beta1 regulation of C-type natriuretic peptide expression in human vascular smooth muscle cells: dependence on TSC22D1.; Authors - Mendon?a Maria C MC
human	WB,IHC	Biochem Biophys Res Commun; Low levels of TSC22 enhance tumorigenesis by inducing cell proliferation in colorectal cancer.; Authors - Yong Zhou