TRPC4AP Polyklonaler Antikörper

TRPC4AP Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 12606-1-AP

Synonyme

C20orf188, Protein TAP1, Protein TRUSS, Trp4 associated protein, Trpc4 associated protein, TRPC4AP, TRRP4AP, TRUSS

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Galerie der Validierungsdaten

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  WB analysis of human heart using 12606-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12606-1-AP (TRPC4AP Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 12606-1-AP

  IP result of anti-TRPC4AP (IP:12606-1-AP, 5ug; Detection:12606-1-AP 1:200) with mouse heart tissue lysate 6000ug.

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  IHC staining of human breast cancer using 12606-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12606-1-AP (TRPC4AP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 12606-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12606-1-AP (TRPC4AP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 12606-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 12606-1-AP (TRPC4AP Antibody) at dilution of 1:50.

• 

  IHC staining of human breast cancer using 12606-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 12606-1-AP (TRPC4AP Antibody) at dilution of 1:50.

• 

  IHC staining of human breast cancer using 12606-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12606-1-AP (TRPC4AP antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Herzgewebe
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

12606-1-AP bindet in WB, IP, IHC, ELISA TRPC4AP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRPC4AP fusion protein Ag3299
• Vollständiger Name: transient receptor potential cation channel, subfamily C, member 4 associated protein
• Berechnetes Molekulargewicht: 797 aa, 91 kDa
• Beobachtetes Molekulargewicht: 85-90 kDa
• GenBank-Zugangsnummer: BC013144
• Gene symbol: TRPC4AP
• Gene ID (NCBI): 26133
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRPC4AP, also named as C20orf188 and TRRP4AP, is a substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control. TRPC4AP participates in the activation of NFKB1 in response to ligation of TNFRSF1A, possibly by linking TNFRSF1A to the IKK signalosome. The calculated molecular weight of TRPC4AP is 91 kDa. Western blot analysis detected a protein of about 82 kDa expressed in several mouse cell lines and in a human promonocytic cell line.

Publikationen

Species	Application	Title
	WB	Genes Dev; Myc protein is stabilized by suppression of a novel E3 ligase complex in cancer cells.; Authors - Seung H Choi
human	WB	Oncogene; PP2A-B56α controls oncogene-induced senescence in normal and tumor human melanocytic cells.; Authors - Mannava S S
	WB	Cell Cycle; The G1 phase E3 ubiquitin ligase TRUSS that gets deregulated in human cancers is a novel substrate of the S-phase E3 ubiquitin ligase Skp2.; Authors - Azfar Jamal