TROAP Polyklonaler Antikörper

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• KD/KO Validated

TROAP Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 13634-1-AP

Synonyme

TASTIN, TROAP, Trophinin assisting protein, Trophinin associated protein

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Galerie der Validierungsdaten

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  WB analysis using 13634-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13634-1-AP (TROAP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13634-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13634-1-AP (TROAP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 13634-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13634-1-AP (TROAP antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 13634-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13634-1-AP (TROAP antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 13634-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13634-1-AP (TROAP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 13634-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13634-1-AP (TROAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HCT 116-Zellen, HT-29-Zellen, Jurkat-Zellen, PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe, Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 8 publications below
• IHC: See 5 publications below

Produktinformation

13634-1-AP bindet in WB, IHC, ELISA TROAP und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TROAP fusion protein Ag4562
• Vollständiger Name: trophinin associated protein (tastin)
• Berechnetes Molekulargewicht: 778 aa, 84 kDa
• Beobachtetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: BC032583
• Gene symbol: TROAP
• Gene ID (NCBI): 10024
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TROAP, also known as tastin, was first cloned from human epithelial cells in 1995. It as a cytoplasmic protein is composed of 778 amino acid residues and contains potential phosphorylation sites for protein kinases. TROAP highly expresses in bone marrow, testis and thymus, and is involved in centrosome integrity and spindle assembly during mitosis (PMID: 33500384). TROAP has been shown to promote some tumor progressions, such as those of breast and colorectal cancers (PMID: 33692939).

Publikationen

Species	Application	Title
		Dev Cell; The HCMV Assembly Compartment Is a Dynamic Golgi-Derived MTOC that Controls Nuclear Rotation and Virus Spread.; Authors - Dean J Procter
human	IHC	Brief Bioinform; Comprehensive assessment of cellular senescence in the tumor microenvironment.; Authors - Xiaoman Wang
human	WB	Biomed Pharmacother; MicroRNA-519d-3p inhibits cell proliferation and migration by targeting TROAP in colorectal cancer.; Authors - Xiaoyong Ye ; KD Validated
human	WB	Cell Cycle; MiR-532-3p suppresses cell viability, migration and invasion of clear cell renal cell carcinoma through targeting TROAP.; Authors - Bin Gao
human	WB	Front Oncol; Downregulation of Methyltransferase-Like 14 Promotes Ovarian Cancer Cell Proliferation Through Stabilizing TROAP mRNA.; Authors - Yize Li
human	WB	CNS Neurosci Ther; TROAP regulates cell cycle and promotes tumor progression through Wnt/β-Catenin signaling pathway in glioma cells.; Authors - Zong-Qing Zhao