TRIP10 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TRIP10 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Rezensionen (1)

Kat-Nr. : 10798-1-AP

Synonyme

TRIP-10, TR-interacting protein 10, Thyroid receptor-interacting protein 10, hSTP, CIP4

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Galerie der Validierungsdaten

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  WB analysis using 10798-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10798-1-AP (TRIP10 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 10798-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10798-1-AP (TRIP10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 10798-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10798-1-AP (TRIP10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of BxPC-3 using 10798-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 10798-1-AP (TRIP10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 10798-1-AP

  IP result of anti-TRIP10 (IP:10798-1-AP, 4ug; Detection:10798-1-AP 1:10000) with K-562 cells lysate 1760 ug.

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  IF Staining of BxPC-3 using 10798-1-AP

  Immunofluorescent analysis of BxPC-3 cells using 10798-1-AP (TRIP10 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 10798-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TRIP10 antibody (10798-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 10798-1-AP

  Immunofluorescent analysis of HeLa cells using 10798-1-AP (TRIP10 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, BxPC-3-Zellen, C6-Zellen, HeLa-Zellen, K-562-Zellen, Maus-Skelettmuskelgewebe, NIH/3T3-Zellen
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, BxPC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

10798-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA TRIP10 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRIP10 fusion protein Ag1251
• Vollständiger Name: thyroid hormone receptor interactor 10
• Berechnetes Molekulargewicht: 63 kDa
• Beobachtetes Molekulargewicht: 75-85 kDa
• GenBank-Zugangsnummer: BC013002
• Gene symbol: TRIP10
• Gene ID (NCBI): 9322
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRIP10 (thyroid hormone receptor interactor 10), also known as CIP4 (Cdc42-interacting protein 4), is a novel adaptor protein which may be invloved in plasma membrane remodeling. CIP4 has been reported to negatively regulate glucose uptake by promoting GLUT4 endocytosis in muscle cells. At least five splice variants for human CIP4 have been identified, including CIP4a (CIP4), CIP4b (FELIC), CIP4c, CIPh (CIP4/2). Western blot analysis usually demonstrates a major band around 75 kDa and a weaker band about 85 kDa.

Publikationen

Species	Application	Title
human	WB,IF	Nat Commun; Mechanical loading of intraluminal pressure mediates wound angiogenesis by regulating the TOCA family of F-BAR proteins.; Authors - Shinya Yuge
human	WB,IHC	J Exp Clin Cancer Res; CDC42-interacting protein 4 promotes metastasis of nasopharyngeal carcinoma by mediating invadopodia formation and activating EGFR signaling.; Authors - Dong-Fang Meng ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

anwesh (Verified Customer) (04-01-2019)

Great antibody. Detects human version of the protein in several cell types. 293T, U2OS and A549 cells tested. Antibody also IP's the endogenous protein

• Applications: Western Blot, Immunoprecipitation,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: 293T

TRIP10 Antibody Western Blot,Immunoprecipitation, validation (1:1000 dilution) in 293T (Cat no:10798-1-AP)